PMID- 9012378
OWN - NLM
STAT- MEDLINE
DCOM- 19970417
LR  - 20191101
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 27
IP  - 2
DP  - 1997 Feb 1
TI  - Methods for automatic multiparameter analysis of fluorescence in situ hybridized 
      specimens with a laser scanning cytometer.
PG  - 117-25
AB  - Multiparameter laser scanning cytometry has been applied to the automatic 
      counting of probe spots and the simultaneous measurement of cellular DNA for 
      fluorescence in situ hybridization (FISH) prepared specimens counterstained with 
      propidium iodide. Relatively low resolution imaging, highly variable probe 
      fluorescence, spectral overlap of probe with counterstain fluorescence, and 
      autofluorescence required the development of an image processing method to detect 
      and isolate FISH probe spots. Inability to properly apportion detected probe 
      spots because of overlapping probe spot images in the same cell required 
      development of a method to eliminate cell data whenever spots in that cell could 
      not be reliably isolated. Laser scanning cytometry incorporating these methods to 
      determine per cell probe spot count and DNA is demonstrated on tissue cultures 
      and peripheral blood cells using different centromeric FISH probes with either 
      FITC or Spectrum Green labeling.
FAU - Kamentsky, L A
AU  - Kamentsky LA
AD  - CompuCyte Corporation, Cambridge, Massachusetts 02139, USA.
FAU - Kamentsky, L D
AU  - Kamentsky LD
FAU - Fletcher, J A
AU  - Fletcher JA
FAU - Kurose, A
AU  - Kurose A
FAU - Sasaki, K
AU  - Sasaki K
LA  - eng
GR  - 5R44CA55413-03/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cells, Cultured
MH  - DNA/analysis
MH  - Female
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Image Cytometry/*methods
MH  - *Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - T-Lymphocytes/cytology
EDAT- 1997/02/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(19970201)27:2<117::AID-CYTO3>3.0.CO;2-D [pii]
AID - 10.1002/(sici)1097-0320(19970201)27:2<117::aid-cyto3>3.0.co;2-d [doi]
PST - ppublish
SO  - Cytometry. 1997 Feb 1;27(2):117-25. doi: 
      10.1002/(sici)1097-0320(19970201)27:2<117::aid-cyto3>3.0.co;2-d.