PMID- 9012447
OWN - NLM
STAT- MEDLINE
DCOM- 19970415
LR  - 20201212
IS  - 0929-1903 (Print)
IS  - 0929-1903 (Linking)
VI  - 4
IP  - 1
DP  - 1997 Jan-Feb
TI  - A comparison of gene transfer methods in human dendritic cells.
PG  - 17-25
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells (APCs) for the 
      initiation of antigen-specific T-cell activation. DCs may be highly enriched from 
      peripheral blood-adherent leukocytes by short-term (7-day) culture in the 
      presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating 
      factor. Various methods of gene transfer were studied, including DNA/liposome 
      complexes, electroporation, CaPO4 precipitation, and recombinant adenovirus (AdV) 
      vectors. Low levels of expression were obtained with the physical methods tested. 
      In contrast, AdV vectors expressing luciferase, beta-galactosidase, IL-2, and 
      IL-7 all readily transduced human DCs. Increasing levels of gene expression were 
      observed over a range of multiplicity of infection (MOI) of 10:1 to 10,000:1, 
      with transduction efficiencies exceeding 95% at higher MOI. Although levels of 
      maximal gene expression in DCs were significantly lower than those obtained using 
      human tumor cell lines, IL-2 and IL-7 production of up to 5 x 10(2) ng/10(6) DC 
      were achieved. These results suggest that AdV vectors are a promising vehicle for 
      genetically engineering human DCs.
FAU - Arthur, J F
AU  - Arthur JF
AD  - Division of Surgical Oncology, University of California at Los Angeles School of 
      Medicine 90095-1782, USA.
FAU - Butterfield, L H
AU  - Butterfield LH
FAU - Roth, M D
AU  - Roth MD
FAU - Bui, L A
AU  - Bui LA
FAU - Kiertscher, S M
AU  - Kiertscher SM
FAU - Lau, R
AU  - Lau R
FAU - Dubinett, S
AU  - Dubinett S
FAU - Glaspy, J
AU  - Glaspy J
FAU - McBride, W H
AU  - McBride WH
FAU - Economou, J S
AU  - Economou JS
LA  - eng
GR  - CA09092-19/CA/NCI NIH HHS/United States
GR  - DA08254/DA/NIDA NIH HHS/United States
GR  - P01CA59326-04/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cancer Gene Ther
JT  - Cancer gene therapy
JID - 9432230
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukin-7)
SB  - IM
MH  - *Dendritic Cells/immunology
MH  - Flow Cytometry
MH  - *Gene Transfer Techniques
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-2/biosynthesis/genetics
MH  - Interleukin-7/biosynthesis/genetics
MH  - Methods
MH  - Recombination, Genetic
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Gene Ther. 1997 Jan-Feb;4(1):17-25.