PMID- 9012775
OWN - NLM
STAT- MEDLINE
DCOM- 19970220
LR  - 20190831
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 34
IP  - 10
DP  - 1996 Oct
TI  - The local lymph node assay: status of validation.
PG  - 999-1002
AB  - For the prediction of skin sensitization potential of substances, the local lymph 
      node assay (LLNA) is an alternative to the widely used guinea pig tests. Over a 
      10-yr period this method has undergone extensive development, evaluation and 
      validation. In this commentary, the quality of this validation is examined. It is 
      concluded that the LLNA has successfully passed through all reasonable validation 
      stages. It provides a reliable and relevant source of predictive skin 
      sensitization data which, unlike results from guinea pig tests, are reproducible 
      from laboratory to laboratory. Thus, it is now ready for acceptance as a viable 
      and complete alternative to traditional methods, offering substantial 
      opportunities for reduction in animal usage and improved animal welfare without 
      compromising standards for the identification of significant skin sensitizers.
FAU - Chamberlain, M
AU  - Chamberlain M
AD  - Unilever Environmental Safety Laboratory, Sharnbrook, Bedford, UK.
FAU - Basketter, D A
AU  - Basketter DA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/*adverse effects/analysis
MH  - Animals
MH  - Humans
MH  - Lymph Nodes/*drug effects/pathology
MH  - Observer Variation
MH  - Reproducibility of Results
MH  - Risk Assessment
MH  - Skin Tests/*standards
RF  - 20
EDAT- 1996/10/01 00:00
MHDA- 1996/10/01 00:01
CRDT- 1996/10/01 00:00
PHST- 1996/10/01 00:00 [pubmed]
PHST- 1996/10/01 00:01 [medline]
PHST- 1996/10/01 00:00 [entrez]
AID - S0278691596000580 [pii]
AID - 10.1016/s0278-6915(96)00058-0 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 1996 Oct;34(10):999-1002. doi: 10.1016/s0278-6915(96)00058-0.