PMID- 9013295
OWN - NLM
STAT- MEDLINE
DCOM- 19970407
LR  - 20190512
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 21
IP  - 1
DP  - 1997 Jan-Feb
TI  - Determination of phenylisothiocyanate derivatives of amphetamine and its 
      analogues in biological fluids by HPLC-APCI-MS or DAD.
PG  - 59-69
AB  - Amphetamine (A), methamphetamine (MA), methylene dioxyamphetamine (MDA), 
      methylenedioxyethylamphelamine (MDE), and methylenedioxymethamphetamine (MDMA), 
      as well as eight other sympathomimetic amines (benzyl-1-phenylethylamine, 
      ephedrine, fenfluramine, norfenfluramine, phentermine, phenylethylamine, 
      phenylpropanolamine, and propylhexedrine), were extracted from serum or urine 
      with ether, derivatized with phenylisothiocyanate, and subjected to 
      high-performance liquid chromatographic (HPLC) examination in isocratic mode. Two 
      detection arts were applied: atmospheric pressure chemical ionization (APCI) mass 
      spectrometry (MS) and UV-spectrometry as diode array detection (DAD) or single 
      wavelength at 250 nm. The derivatives were well-separated and showed good 
      chromatographic behavior. Full-scan mass spectra of drugs examined by means of 
      APCI with collision induced dissociation (APCID) contained protonated molecular 
      ions (M+H)+ and fragments typical for particular drugs. APCID-liquid 
      chromatography-mass spectrometry (LC-MS) appeared very selective for 
      differentiation of all drugs involved. The quantitation with APCID was performed 
      using selected ion monitoring (SIM) of (M+H)+ ions and selected fragments of 
      drugs involved and their deuterated analogues. The limits of detection ranged 
      from 0.001 mg/L (MA, MDMA, and MDE) to 0.005 mg/L (A and MDA). In HPLC-DAD, the 
      spectra of MDMA and MDE were practically identical with maxima of 236-240 nm. 
      Other amphetamines showed slightly different spectra with maxima of 245-250 nm. 
      The limits of detection in UV detection amounted to 0.01-0.03 mg/L (single 
      wavelength detector at 250 nm) or 0.05-0.1 mg/L (DAD).
FAU - Bogusz, M J
AU  - Bogusz MJ
AD  - Institute of Forensic Medicine, Aachen University of Technology, Germany.
FAU - Kala, M
AU  - Kala M
FAU - Maier, R D
AU  - Maier RD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Isothiocyanates)
RN  - 0 (Sympathomimetics)
RN  - 0 (Thiocyanates)
RN  - 0D58F84LSU (phenylisothiocyanate)
SB  - IM
MH  - Amphetamines/*analysis/chemistry
MH  - Body Fluids/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Forensic Medicine/methods
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Isothiocyanates
MH  - Sensitivity and Specificity
MH  - Substance Abuse Detection/*methods
MH  - Sympathomimetics/*analysis/chemistry
MH  - Thiocyanates/*chemistry
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1093/jat/21.1.59 [doi]
PST - ppublish
SO  - J Anal Toxicol. 1997 Jan-Feb;21(1):59-69. doi: 10.1093/jat/21.1.59.