PMID- 9013945
OWN - NLM
STAT- MEDLINE
DCOM- 19970219
LR  - 20071114
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 158
IP  - 3
DP  - 1997 Feb 1
TI  - Fc(gamma) receptor cross-linking induces peripheral blood mononuclear cell 
      monocyte chemoattractant protein-1 expression: role of lymphocyte Fc(gamma)RIII.
PG  - 1078-84
AB  - Immune complexes activate cells by cross-linking leukocyte surface Fc(gamma)Rs. 
      Diseases associated with immune complex deposition, such as rheumatoid arthritis, 
      glomerulonephritis, or idiopathic pulmonary fibrosis, are characterized by 
      compartmentalized monocyte infiltration. The factors that recruit monocytes to 
      these compartments are not well characterized; however, monocyte chemoattractant 
      protein-1 (MCP-1) has been found in areas of tissue injury. To account for these 
      observations we hypothesized that PBMC Fc(gamma)R cross-linking may induce MCP-1 
      synthesis, which stimulates further monocyte recruitment. To test this 
      hypothesis, PBMC were incubated on increasing concentrations of immobilized human 
      IgG, a stimulus for Fc(gamma)R cross-linking. Immunoreactive MCP-1 was produced 
      in a dose-dependent manner (p < 0.0001). MCP-1 was specifically induced by 
      Fc(gamma)R cross-linking, since immobilized F(ab')2 fragments of human IgG did 
      not activate MCP-1 production. This effect was reproduced by directly 
      cross-linking PBMC Fc(gamma)RIII, but not by cross-linking Fc(gamma)RI or 
      Fc(gamma)RII. PBMC-derived MCP-1 stimulated monocyte chemotaxis that was 
      inhibited by a neutralizing anti-MCP-1 Ab. MCP-1 levels correlated with increased 
      PBMC mRNA expression. Interestingly, Fc(gamma)R cross-linking with either 
      immobilized IgG or anti-Fc(gamma)RIII induced more MCP-1 release from PBMC than 
      from autologous monocytes (p = 0.02). Lymphocytes, the main cell type found in 
      PBMC preparations, did not independently produce a significant amount of MCP-1, 
      but when incubated on immobilized IgG or anti-Fc(gamma)RIII secreted a soluble 
      factor(s) that induced monocyte MCP-1 production. These data suggest that 
      cross-linking PBMC Fc(gamma)R induces the production of bioactive MCP-1. This 
      occurs in part at the level of gene transcription and involves a cooperative 
      interaction between monocytes and lymphocytes.
FAU - Marsh, C B
AU  - Marsh CB
AD  - Division of Pulmonary and Critical Care Medicine, Ohio State University, Columbus 
      43210, USA.
FAU - Wewers, M D
AU  - Wewers MD
FAU - Tan, L C
AU  - Tan LC
FAU - Rovin, B H
AU  - Rovin BH
LA  - eng
GR  - DK46055/DK/NIDDK NIH HHS/United States
GR  - HL40871/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Humans
MH  - Immunoglobulin G/*immunology
MH  - Lymphocytes/*immunology
MH  - Monocytes/*metabolism
MH  - Receptor Aggregation
MH  - Receptors, IgG/*physiology
MH  - Signal Transduction
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1997 Feb 1;158(3):1078-84.