PMID- 9014819
OWN - NLM
STAT- MEDLINE
DCOM- 19970220
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 89
IP  - 4
DP  - 1996 Dec
TI  - Interferon-gamma promotes the survival and Fc epsilon RI-mediated histamine 
      release in cultured human mast cells.
PG  - 547-52
AB  - We examined the effects of interferon-gamma (IFN-gamma) on 100% pure human mast 
      cells generated in suspension cultures of umbilical cord blood mononuclear cells 
      in the presence of stem cell factor (SCF) and interleukin-6 (IL-6). When mast 
      cells were suspended in serum-free medium without any cytokine after the 
      withdrawal of SCF and IL-6, they died over a period of 5 days because of 
      apoptosis. IFN-gamma in the cultures suppressed apoptosis and prolonged their 
      survival in a dose-dependent manner. This survival-promoting effect of IFN-gamma 
      was blocked by neutralizing antibodies to IFN-gamma or to IFN-gamma receptor 
      (IFN-gamma R). When mast cells were incubated with IFN-gamma in serum-free medium 
      for more than 4 hr during sensitization, immunoglobulin E (IgE)/anti-IgE 
      antibody-induced histamine release was effectively enhanced. Polymerase chain 
      reaction (PCR) amplification of the alpha-chain of IFN-gamma R (IFN-gamma R 
      alpha) yielded products of the correct size predicted from the sequence of the 
      receptor. In addition, flow cytometry using anti-IFN-gamma R monoclonal 
      antibodies (mAbs) indicated that these mast cells bear IFN-gamma R on their 
      surface. These findings suggested that IFN-gamma activates human mast cells via 
      specific receptors in certain aspects of inflammatory reactions.
FAU - Yanagida, M
AU  - Yanagida M
AD  - Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd, Gunma, Japan.
FAU - Fukamachi, H
AU  - Fukamachi H
FAU - Takei, M
AU  - Takei M
FAU - Hagiwara, T
AU  - Hagiwara T
FAU - Uzumaki, H
AU  - Uzumaki H
FAU - Tokiwa, T
AU  - Tokiwa T
FAU - Saito, H
AU  - Saito H
FAU - Iikura, Y
AU  - Iikura Y
FAU - Nakahata, T
AU  - Nakahata T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, Interferon)
RN  - 0 (Stem Cell Factor)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Apoptosis/*drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Histamine Release/*immunology
MH  - Humans
MH  - Interferon-gamma/immunology/*pharmacology
MH  - Interleukin-6/metabolism
MH  - Mast Cells/drug effects/*physiology
MH  - Polymerase Chain Reaction
MH  - Receptors, IgE/immunology
MH  - Receptors, Interferon/immunology
MH  - Stem Cell Factor/metabolism
MH  - Stimulation, Chemical
PMC - PMC1456572
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
PMCR- 1997/12/01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PHST- 1997/12/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1996.d01-768.x [doi]
PST - ppublish
SO  - Immunology. 1996 Dec;89(4):547-52. doi: 10.1046/j.1365-2567.1996.d01-768.x.