PMID- 9015294
OWN - NLM
STAT- MEDLINE
DCOM- 19970415
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 63
IP  - 1-2
DP  - 1997 Jan
TI  - Detection of porcine reproductive and respiratory syndrome virus in 
      paraffin-embedded tissues: comparison of immunohistochemistry and in situ 
      hybridization.
PG  - 227-35
AB  - A non-radioactive in situ hybridization (ISH) method developed recently and an 
      immunohistochemical method, the immunogold silver staining (IGSS), were compared 
      for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) 
      in formalin-fixed paraffin-embedded tissues. Serial sections from 98 tissues, 
      representing different organs from PRRSV experimentally infected pigs, and serial 
      sections from 46 lung tissues from field cases were tested by both methods. 
      Results obtained on tissues from experimentally infected pigs and tissues from 
      field cases demonstrated that ISH was more sensitive than immunohistochemistry. 
      More tissues were positive by ISH compared to IGSS and also a greater number of 
      labelled cells and a stronger signal in stained cells were observed in 
      ISH-treated sections. The ISH method described, using a 254 bp 
      digoxigenin-labelled cDNA probe, is a rapid, highly specific and sensitive 
      detection method which can be used for the diagnosis of PRRSV in routinely fixed 
      and processed tissues.
FAU - Larochelle, R
AU  - Larochelle R
AD  - Laboratoire d'hygiene veterinaire et alimentaire, Agriculture et Agroalimentaire 
      Canada, Saint-Hyacinthe, Quebec. larocheller@em.agr.ca
FAU - Magar, R
AU  - Magar R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Animals
MH  - *Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - Nucleocapsid/analysis
MH  - Paraffin Embedding
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Swine
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0166-0934(96)02131-3 [pii]
AID - 10.1016/s0166-0934(96)02131-3 [doi]
PST - ppublish
SO  - J Virol Methods. 1997 Jan;63(1-2):227-35. doi: 10.1016/s0166-0934(96)02131-3.