PMID- 9016759
OWN - NLM
STAT- MEDLINE
DCOM- 19970305
LR  - 20181130
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 230
IP  - 2
DP  - 1997 Jan 13
TI  - Possible involvement of Bcl-2 pathway in retinoid X receptor alpha-induced 
      apoptosis of HL-60 cells.
PG  - 251-3
AB  - Retinoids induce granulocytic differentiation and subsequent apoptosis in human 
      myeloid (HL-60) leukemia cells. Differentiation is induced due to activation of 
      retinoic acid receptors (RARs) whereas, activation of retinoid X receptors (RXRs) 
      seems to be essential for driving these cells into apoptosis. In order to 
      understand the mechanism of RXR induced apoptosis, we used a variant HL-60 cell 
      line (HL-60R) with a transdominant negative mutation. The retroviral 
      vector-mediated gene transfer was used to introduce the functional RARs or RXR 
      alpha into HL-60R cells. We studied the effect of receptor-selective retinoid 
      treatment on the expression of Bcl-2 and Bax oncogenes by reverse-transcription 
      polymerase chain reaction (RT-PCR) and immunoblot analysis in RARs and RXR alpha 
      transfected HL-60 cells. Our results show that activation of RXR alpha results in 
      apoptosis via down-modulation of Bcl-2 mRNA as well as its gene product 
      expression with no change in Bax mRNA expression.
FAU - Agarwal, N
AU  - Agarwal N
AD  - Department of Anatomy and Cell Biology, University of North Texas Health Science 
      Center and North Texas Eye Research Institute, Fort Worth 76107, USA. 
      nagarwal@molly.hsc.unt.edu
FAU - Mehta, K
AU  - Mehta K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (BAX protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RARA protein, human)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Retinoic Acid Receptor alpha)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 1UA8E65KDZ (Alitretinoin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Alitretinoin
MH  - *Apoptosis
MH  - HL-60 Cells
MH  - Humans
MH  - Mutation
MH  - Oncogenes
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins/biosynthesis
MH  - Proto-Oncogene Proteins c-bcl-2/*biosynthesis
MH  - Receptors, Retinoic Acid/biosynthesis/*physiology
MH  - Recombinant Proteins/biosynthesis
MH  - Retinoic Acid Receptor alpha
MH  - Retinoid X Receptors
MH  - Retroviridae
MH  - Transcription Factors/biosynthesis/*physiology
MH  - *Transcription, Genetic/drug effects
MH  - Transfection
MH  - Tretinoin/*pharmacology
MH  - bcl-2-Associated X Protein
EDAT- 1997/01/13 00:00
MHDA- 1997/01/13 00:01
CRDT- 1997/01/13 00:00
PHST- 1997/01/13 00:00 [pubmed]
PHST- 1997/01/13 00:01 [medline]
PHST- 1997/01/13 00:00 [entrez]
AID - S0006-291X(96)95937-9 [pii]
AID - 10.1006/bbrc.1996.5937 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1997 Jan 13;230(2):251-3. doi: 
      10.1006/bbrc.1996.5937.