PMID- 9016861
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20191101
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 129
IP  - 2
DP  - 1997 Feb
TI  - Expression of monocyte chemoattractant protein-1 in experimental crescentic 
      glomerulonephritis in rats.
PG  - 239-44
AB  - Crescentic glomerulonephritis (CGN) is a rapidly progressive glomerular disease 
      that is usually associated with a poor prognosis. Monocytes/macrophages are 
      frequently observed in glomeruli in cases of CGN, and they are considered to play 
      a crucial role in the pathogenesis of this disease. In this study we analyzed the 
      glomerular expression of monocyte chemoattractant protein-1 (MCP-1), a potent 
      chemoattractant for monocytes, in an experimental model of CGN. A model of the 
      disease was induced in the WKY strain of rats by intravenous injection of 
      antiserum raised against glomerular basement membranes. Accumulation of 
      monocytes/macrophages in glomeruli was observed 4 hours after the injection of 
      antiserum. Northern blot analysis showed that the expression of mRNA for MCP-1 
      was enhanced within 4 hours, peaked on day 3--when it was 60 times that in the 
      control--and then declined. Immunostaining with MCP-1-specific antibody revealed 
      the expression of MCP-1 protein in the diseased glomeruli but not in control 
      glomeruli. Quantitative analysis of glomerular MCP-1 protein by enzyme-linked 
      immunosorbent assay revealed a level 46 times that in the control in reflecting 
      the increase in mRNA for MCP-1. These results indicate that glomeruli of rats 
      with CGN produce MCP-1, which may play an important role in the pathogenesis of 
      glomerular inflammation and crescent formation in CGN.
FAU - Sekiguchi, M
AU  - Sekiguchi M
AD  - Division of Pathophysiology, Research Institute, International Medical Center of 
      Japan, Tokyo.
FAU - Natori, Y
AU  - Natori Y
FAU - Iyonaga, K
AU  - Iyonaga K
FAU - Takeya, M
AU  - Takeya M
FAU - Natori, Y
AU  - Natori Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immune Sera)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Basement Membrane/immunology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glomerulonephritis/immunology/*metabolism/*pathology
MH  - Immune Sera/immunology
MH  - Kidney Glomerulus/immunology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred WKY
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0022-2143(97)90145-1 [pii]
AID - 10.1016/s0022-2143(97)90145-1 [doi]
PST - ppublish
SO  - J Lab Clin Med. 1997 Feb;129(2):239-44. doi: 10.1016/s0022-2143(97)90145-1.