PMID- 9020313
OWN - NLM
STAT- MEDLINE
DCOM- 19970307
LR  - 20191024
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 29
IP  - 1
DP  - 1997
TI  - Methods for improving the yield and quality of metaphase preparations for FISH 
      probing of human lymphocyte chromosomes.
PG  - 98-104
AB  - Procedures are described for the in vitro culture of human lymphocytes, which 
      have been concentrated by density gradient centrifugation, and for a modified 
      slide-making technique for the fixed cells. The method yields improved 
      percentages of mitotic cells which are largely synchronized at harvest. 
      Controlled placement of fixed cells on slides produces well-spread metaphase 
      preparations with little background material to interfere with fluorescence in 
      situ hybridization (FISH) probe procedures. The FISH reagents and microscope 
      scanning time required are minimized by concentrating cells in a defined area of 
      the slide.
FAU - McFee, A F
AU  - McFee AF
AD  - Environmental and Health Sciences Division, Oak Ridge Institute for Science and 
      Education, Tennessee 37831-0117, USA.
FAU - Sayer, A M
AU  - Sayer AM
FAU - Salomaa, S I
AU  - Salomaa SI
FAU - Lindholm, C
AU  - Lindholm C
FAU - Littlefield, L G
AU  - Littlefield LG
LA  - eng
GR  - CA51388/CA/NCI NIH HHS/United States
GR  - N01-CP-40517/CP/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
SB  - IM
MH  - Cell Culture Techniques/*methods
MH  - Cryopreservation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/*cytology
MH  - *Metaphase
MH  - Mitotic Index
MH  - Reproducibility of Results
MH  - Specimen Handling
MH  - Staining and Labeling/*methods
MH  - Time Factors
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1997)29:1<98::AID-EM13>3.0.CO;2-C [pii]
AID - 10.1002/(sici)1098-2280(1997)29:1<98::aid-em13>3.0.co;2-c [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1997;29(1):98-104. doi: 
      10.1002/(sici)1098-2280(1997)29:1<98::aid-em13>3.0.co;2-c.