PMID- 9020486
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 75
IP  - 3
DP  - 1997
TI  - Interphase fluorescence in situ hybridization improves the detection of malignant 
      cells in effusions from breast cancer patients.
PG  - 403-7
AB  - In diagnostic evaluation of effusions, difficulties are encountered when atypical 
      reactive mesothelial cells have to be differentiated from malignant cells. We 
      tested the impact of fluorescence in situ hybridization (FISH) to identify 
      metastatic cells in breast cancer effusions by detection of numerical chromosomal 
      changes. Pleural and ascitic fluid samples (n=57) from 41 breast cancer patients 
      were concomitantly evaluated by routine cytology and FISH, using 
      centromere-specific probes representing chromosomes 7, 11, 12, 17 and 18. After 
      setting stringent cut-off levels deduced from non-malignant control effusions 
      (n=9), the rates of cells with true aneuploidy were determined in each effusion 
      sample from breast cancer patients. The occurrence of aneuploid cells, as 
      detected by FISH and indicative of malignancy, was correlated with the 
      cytological findings. Routine cytology revealed malignancy in 60% of effusions. 
      Using FISH, aneuploid cell populations could be observed in 94% of cytologically 
      positive and in 48% of cytologically negative effusions, thus reverting diagnosis 
      to malignancy. To confirm malignancy in cases with a low frequency of aneuploid 
      cells, two-colour FISH was additionally performed and indeed showed heterogeneous 
      chromosomal aneuploidy within single nuclei. We conclude that FISH is a valuable 
      tool in the diagnosis of malignancy and may serve as an adjunct to routine 
      cytological examination, as demonstrated here for breast cancer effusions.
FAU - Zojer, N
AU  - Zojer N
AD  - First Department of Internal Medicine, Division of Clinical Oncology, University 
      of Vienna, Austria.
FAU - Fiegl, M
AU  - Fiegl M
FAU - Angerler, J
AU  - Angerler J
FAU - Mullauer, L
AU  - Mullauer L
FAU - Gsur, A
AU  - Gsur A
FAU - Roka, S
AU  - Roka S
FAU - Pecherstorfer, M
AU  - Pecherstorfer M
FAU - Huber, H
AU  - Huber H
FAU - Drach, J
AU  - Drach J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aneuploidy
MH  - Ascites/*pathology
MH  - Breast Neoplasms/genetics/*pathology
MH  - Centromere
MH  - Chromosome Mapping
MH  - *Chromosomes, Human
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Pleural Effusion/*pathology
PMC - PMC2063365
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1038/bjc.1997.65 [doi]
PST - ppublish
SO  - Br J Cancer. 1997;75(3):403-7. doi: 10.1038/bjc.1997.65.