PMID- 9021370
OWN - NLM
STAT- MEDLINE
DCOM- 19970410
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 11
IP  - 12
DP  - 1996 Dec
TI  - Cell-specific localization of nitric oxide synthases (NOS) in the rat ovary 
      during follicular development, ovulation and luteal formation.
PG  - 2667-73
AB  - Nitric oxide (NO) has emerged as one of several important intraovarian regulatory 
      factors. In particular, NO has been implicated in the processes of ovulation and 
      atresia-related apoptosis. The aim of the present study was to investigate the 
      presence and distribution of the NO-generating nitric oxide synthase (NOS) 
      enzymes in the ovary during follicular development, ovulation and luteal 
      formation of the equine chorionic gonadotrophin (ECG)/human chorionic 
      gonadotrophin (HCG)-primed rat. NADPH diaphorase activity was used as a 
      histochemical marker for NOS within the ovary. Diaphorase reactivity was most 
      abundant in the stroma (S) of the ovary and in the theca (T) layer of the 
      follicle. In luteinized ovaries, weaker diaphorase reactivity was present within 
      the corpora lutea (CL). Two different isoforms of NOS, the constitutively 
      expressed endothelial NOS (eNOS) and the inducible isoform of NOS (iNOS), were 
      immunolocalized in ovaries of immature rats and in ECG/HCG-primed rats during the 
      periovulatory period from HCG injection until 2 days after ovulation. In 
      addition, ovarian concentrations of eNOS and iNOS were quantified by 
      immunoblotting. Immunoblotting with a monoclonal anti-eNOS antibody demonstrated 
      the presence of eNOS mainly in the residual ovary (ROV) during the periovulatory 
      period. In luteinized ovaries, higher concentrations of eNOS were seen in CL, 
      while those in the ROV at this stage were lower than in the periovulatory ovary. 
      Immature ovaries contained diminutive amounts of eNOS, detectable mostly in the 
      ROV compartment. In contrast, iNOS was barely detectable during follicular 
      development to the preovulatory stage. A slight elevation of iNOS was observed in 
      the granulosa cells at 6 h after the HCG injection. The levels of iNOS during the 
      luteal phase were also low. Immunohistochemical analysis using polyclonal eNOS 
      and iNOS antibodies revealed the localization of these two isoforms primarily in 
      the S and the T of the periovulatory ovary. In luteinized ovaries, positive 
      immunoreactivity was also seen within the CL. With a monoclonal antibody against 
      eNOS, intense immunoreactivity was observed in the S, T and within CL. There was 
      a particularly strong staining in blood vessels. These data demonstrate the 
      presence of an intraovarian NO-generating system. The localization of this system 
      to the S, T and CL suggests a role for NO in the ovulatory process and in the 
      regulation of CL function.
FAU - Zackrisson, U
AU  - Zackrisson U
AD  - Department of Obstetrics and Gynaecology, Goteborg University, Sahlgrenska 
      sjukhuset, Sweden.
FAU - Mikuni, M
AU  - Mikuni M
FAU - Wallin, A
AU  - Wallin A
FAU - Delbro, D
AU  - Delbro D
FAU - Hedin, L
AU  - Hedin L
FAU - Brannstrom, M
AU  - Brannstrom M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Gonadotropins)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
SB  - IM
MH  - Animals
MH  - Chorionic Gonadotropin/pharmacology
MH  - Corpus Luteum/*physiology
MH  - Endothelium, Vascular/enzymology
MH  - Female
MH  - Gonadotropins/pharmacology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - NADPH Dehydrogenase/analysis
MH  - Nitric Oxide Synthase/*analysis
MH  - Ovarian Follicle/*physiology
MH  - Ovary/*enzymology
MH  - Ovulation/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stromal Cells/enzymology
MH  - Theca Cells/enzymology
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a019189 [doi]
PST - ppublish
SO  - Hum Reprod. 1996 Dec;11(12):2667-73. doi: 10.1093/oxfordjournals.humrep.a019189.