PMID- 9023107
OWN - NLM
STAT- MEDLINE
DCOM- 19970414
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 25
IP  - 5
DP  - 1997 Mar 1
TI  - Wild-type but not mutant p53 activates the hepatocyte growth factor/scatter 
      factor promoter.
PG  - 983-6
AB  - p53 transactivates the expression of a variety of genes by binding to specific 
      DNA sequences within the promoter. We have investigated the ability of wild-type 
      p53 and a non-DNA binding p53 mutant to activate the hepatocyte growth 
      factor/scatter factor (HGF/SF) promoter using chloramphenicol acetyltransferase 
      reporter constructs. We also used deletion sequences of the HGF/SF promoter to 
      identify which regions, if any, were responsible for p53 binding. Our results 
      show that wild-type but not mutant p53 activates the HGF/SF promoter when using 
      -3000 and -755 bp upstream of the HGF/SF gene. This activation is lost when 
      promoter sequences covering -365 and -239 bp are used. Analysis of the DNA 
      sequence between -365 and -755 bp shows one putative p53 half-site with 80% 
      homology to the consensus sequence and another half-site 3 bases downstream of 
      this with 100% homology to the consensus sequence. In contrast to previously 
      identified p53 binding DNA sequences, the downstream half-site is inverted. We 
      propose that the HGF/SF promoter can be activated by wild-type p53 in vivo and 
      that this could be as a result of a novel form of sequence-specific DNA binding.
FAU - Metcalfe, A M
AU  - Metcalfe AM
AD  - MRC Clinical and Biochemical Magnetic Resonance Unit, Department of Biochemistry, 
      University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
FAU - Dixon, R M
AU  - Dixon RM
FAU - Radda, G K
AU  - Radda GK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Binding Sites
MH  - Cell Line, Transformed
MH  - *Gene Expression Regulation
MH  - Hepatocyte Growth Factor/*genetics
MH  - Humans
MH  - Mice
MH  - Mutation
MH  - *Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/genetics
MH  - *Transcriptional Activation
MH  - Tumor Suppressor Protein p53/genetics/*physiology
PMC - PMC146535
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
PMCR- 1997/03/01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
PHST- 1997/03/01 00:00 [pmc-release]
AID - gka176 [pii]
AID - 10.1093/nar/25.5.983 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1997 Mar 1;25(5):983-6. doi: 10.1093/nar/25.5.983.