PMID- 9023395
OWN - NLM
STAT- MEDLINE
DCOM- 19970306
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 28
IP  - 2
DP  - 1997 Feb
TI  - Detection of the Mbcr/abl translocation in chronic myeloid leukemia by 
      fluorescence in situ hybridization: comparison with conventional cytogenetics and 
      implications for minimal residual disease detection.
PG  - 154-9
AB  - The correlation between the detection of the Philadelphia chromosome by 
      conventional cytogenetics and the identification of Mbcr/abl translocation by 
      fluorescence in situ hybridization (FISH) in both metaphase and interphase cells 
      is prospectively analyzed in a group of 21 chronic myeloid leukemia (CML) 
      patients. To gain insight into the sensitivity and specificity of the detection 
      of the bcr/abl translocation by FISH, a group of 10 healthy volunteers was also 
      studied. Our results show that for the detection of bcr/abl translocation in CML 
      patients, FISH is more sensitive than conventional cytogenetics because it 
      detects significantly higher proportions of cells carrying the translocation both 
      in metaphase (P < .0002) and interphase nuclei (P < .003). Moreover, in the 
      metaphases of the controls analyzed, no bcr/abl+ chromosome was detected that 
      makes the colocalization of bcr and abl signals in the CML patients highly 
      specific. Conversely, in control interphase nuclei, a small proportion of cells 
      (ranging between 0% and 3%, mean value of 1.7% +/- 0.9%) displaying 
      colocalization of both signals is usually detected. This limits, at least for the 
      moment, the routine use of FISH for the detection of minimal residual disease in 
      CML patients at levels lower than 10(-1).
FAU - Garcia-Isidoro, M
AU  - Garcia-Isidoro M
AD  - Departamento de Biologia Celular, Universidad de Salamanca, Spain.
FAU - Tabernero, M D
AU  - Tabernero MD
FAU - Garcia, J L
AU  - Garcia JL
FAU - Najera, M L
AU  - Najera ML
FAU - Hernandez, J M
AU  - Hernandez JM
FAU - Wiegant, J
AU  - Wiegant J
FAU - Raap, A
AU  - Raap A
FAU - San Miguel, J
AU  - San Miguel J
FAU - Orfao, A
AU  - Orfao A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Cell Count
MH  - Chromosomes, Human, Pair 22
MH  - Chromosomes, Human, Pair 9
MH  - *Cytogenetics
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Genes, abl/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis
MH  - Male
MH  - Middle Aged
MH  - Philadelphia Chromosome
MH  - *Translocation, Genetic
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0046-8177(97)90099-X [pii]
AID - 10.1016/s0046-8177(97)90099-x [doi]
PST - ppublish
SO  - Hum Pathol. 1997 Feb;28(2):154-9. doi: 10.1016/s0046-8177(97)90099-x.