PMID- 9024972 OWN - NLM STAT- MEDLINE DCOM- 19970403 LR - 20200304 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 4 IP - 8 DP - 1996 Dec TI - Ribosomal RNA location in the Antarctic fish Champsocephalus gunnari (Notothenioidei, Channichthyidae) using banding and fluorescence in situ hybridization. PG - 557-61 AB - A biotinylated 28S rDNA probe was prepared from the genomic DNA of the Antarctic ice-fish Champsocephalus gunnari and hybridized to metaphase chromosomes of the same species by fluorescence in situ hybridization (FISH). The hybridization signal appeared over the whole heterochromatic arm of the submetacentric chromosomes bearing the nucleolar organizer regions. The results of rDNA/FISH are compared with those coming from classical cytogenetic (C, Q, Ag-NOR, chromomycin A3) banding techniques. The in situ detection of a specific DNA sequence offers a new more precise perspective for understanding the evolving process in chromosomes of Antarctic fish and will provide an interesting contribution to comparative cytogenetics of lower vertebrates. FAU - Ozouf-Costaz, C AU - Ozouf-Costaz C AD - Museum National d'Histoire Naturelle, Laboratoire d'Ichthyologie, CNRS GDR 1005, Paris, France. ozouf@mnhn.fr. FAU - Pisano, E AU - Pisano E FAU - Bonillo, C AU - Bonillo C FAU - Williams, R AU - Williams R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (RNA, Ribosomal, 28S) SB - IM EIN - Chromosome Res 1997 Apr;5(2):152 MH - Animals MH - Chromosome Banding MH - *Chromosome Mapping MH - Female MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Male MH - Perciformes/*genetics MH - RNA, Ribosomal, 28S/*genetics EDAT- 1996/12/01 00:00 MHDA- 1996/12/01 00:01 CRDT- 1996/12/01 00:00 PHST- 1996/12/01 00:00 [pubmed] PHST- 1996/12/01 00:01 [medline] PHST- 1996/12/01 00:00 [entrez] AID - 10.1007/BF02261718 [doi] PST - ppublish SO - Chromosome Res. 1996 Dec;4(8):557-61. doi: 10.1007/BF02261718.