PMID- 9024975
OWN - NLM
STAT- MEDLINE
DCOM- 19970403
LR  - 20220317
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 4
IP  - 8
DP  - 1996 Dec
TI  - Characterization of the translocated chromosome using fluorescence in situ 
      hybridization and random amplified polymorphic DNA on two Triticum 
      aestivum-Thinopyrum intermedium translocation lines resistant to wheat streak 
      mosaic or barley yellow dwarf virus.
PG  - 583-7
AB  - Fluorescence in situ hybridization (FISH) was used to determine the breakpoint of 
      the translocation chromosome in two bread wheat (Triticum aestivum) germplasm 
      lines with Thinopyrum intermedium chromatin carrying resistance to either wheat 
      streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, 
      genome-specific random amplified polymorphic DNA (RAPD) markers were used to 
      ascertain the genomic sources of the Th. intermedium chromosome carrying the WSMV 
      or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from 
      induced homoeologous pairing by using the ph1b mutant, had a translocation 
      chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, 
      and the entire 4ES of Th. intermedium. The BYDV-resistant translocation line, 
      TC14, derived from tissue culture, had a very short distal segment of 7StL from 
      Th. intermedium terminally attached to 56% of the proximal 7DL. These 
      observations indicate that translocations in these wheat germplasm lines did not 
      involve centromeric breaks and fusion but were a result of homoeologous 
      chromosomes recombination.
FAU - Wang, R R
AU  - Wang RR
AD  - USDA-ARS (United States Department of Agriculture-Agricultural Research Service), 
      Forage and Range Research Laboratory, Utah State University, Logan 84322-6300, 
      USA. rrcwang@cc.usu.edu
FAU - Zhang, X Y
AU  - Zhang XY
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Culture Techniques
MH  - DNA
MH  - In Situ Hybridization, Fluorescence
MH  - Mosaic Viruses/*physiology
MH  - Poaceae/*genetics/virology
MH  - Polymorphism, Genetic
MH  - Potyvirus/*physiology
MH  - Random Amplified Polymorphic DNA Technique
MH  - *Translocation, Genetic
MH  - Triticum/*genetics/virology
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - 10.1007/BF02261721 [doi]
PST - ppublish
SO  - Chromosome Res. 1996 Dec;4(8):583-7. doi: 10.1007/BF02261721.