PMID- 9027739
OWN - NLM
STAT- MEDLINE
DCOM- 19970519
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 51
IP  - 2
DP  - 1997 Feb
TI  - Hypoxia-inducible factor-1 alpha is regulated at the post-mRNA level.
PG  - 560-3
AB  - The hypoxia-inducible factor-1 (HIF-1) is involved in the induction of oxygen 
      regulated genes such as erythropoietin and vascular endothelial growth factor 
      (VEGF). HIF-1 is a heterodimeric transcription factor consisting of an alpha and 
      a beta subunit. The question of how HIF-1 itself is regulated remains to be 
      elucidated. Studies performed in human Hep3B hepatoma cells suggested that the 
      prevalent mode of HIF-1 action is an increase in HIF-1 alpha steady-state mRNA 
      and protein levels after hypoxic exposure. In contrast to the reported very low 
      basal HIF-1 alpha mRNA levels, however, we detected HIF-1 alpha mRNA in several 
      cell lines cultured under normoxic conditions, although no HIF-1 DNA binding 
      activity was observed. Following hypoxic induction, VEGF mRNA levels and HIF-1 
      DNA binding activity increased, but HIF-1 alpha mRNA levels remained largely 
      unchanged. One possible explanation for this discrepancy might be that HIF-1 DNA 
      binding activity does not follow HIF-1 alpha mRNA kinetics. We therefore 
      incubated HeLaS3 cells in tonometers for 7.5 minutes up to four hours at either 
      20% O2 or 0.5% O2. Although there was some variation in HIF-1 alpha mRNA levels, 
      we did not find significant changes over this time frame, suggesting that HIF-1 
      alpha is not transcriptionally regulated.
FAU - Wenger, R H
AU  - Wenger RH
AD  - Institute of Physiology, University of Zurich-Irchel, Switzerland.
FAU - Kvietikova, I
AU  - Kvietikova I
FAU - Rolfs, A
AU  - Rolfs A
FAU - Gassmann, M
AU  - Gassmann M
FAU - Marti, H H
AU  - Marti HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Lymphokines)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia/genetics/physiology
MH  - Cell Line
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/chemistry/*genetics/*metabolism
MH  - Endothelial Growth Factors/genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Kinetics
MH  - Lymphokines/genetics
MH  - Mice
MH  - Nuclear Proteins/chemistry/*genetics/*metabolism
MH  - RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/*genetics/metabolism
MH  - *Transcription Factors
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0085-2538(15)59919-5 [pii]
AID - 10.1038/ki.1997.79 [doi]
PST - ppublish
SO  - Kidney Int. 1997 Feb;51(2):560-3. doi: 10.1038/ki.1997.79.