PMID- 9028305
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 89
IP  - 3
DP  - 1997 Feb 1
TI  - Identification of the cDNA for human red blood cell-specific hexokinase isozyme.
PG  - 762-6
AB  - A unique cDNA for hexokinase (HK) was identified from poly(A)+ RNA of human 
      reticulocytes by anchored polymerase chain reaction. This appeared to represent 
      the cDNA for the red blood cell (RBC)-specific HK isozyme (HKR) described in our 
      previous study (Murakami et al: Blood 75:770, 1990). Its nucleotide sequence was 
      identical to HKI cDNA except for the 5' extreme end. It lacked the first 62 
      nucleotides of the HKI coding region: instead, it contained a unique sequence of 
      60 nucleotides at the beginning of the coding sequence as well as another unique 
      sequence upstream of the putative translation initiation site. It lacked the 
      porin-binding domain which facilitates binding to the mitochondria, thus 
      explaining the exclusive cytoplasmic localization of HKR. It was the major cDNA 
      derived from reticulocytes, consistent with the observation that HKR activity is 
      predominant in reticulocytes. Northern blot analysis showed that it was expressed 
      in the reticulocytes and in the K562 erythroleukemic cell line, but not in a 
      lymphocytic cell line. In the extract of K562 cells, HKR activity co-eluted with 
      the HKR of human RBCs on a MonoQ column (Pharmacia, Piscataway, NJ) 
      chromatography, using a salt gradient elution. The separate genetic control of 
      the RBC-specific HK isozyme explains the clinical reports of two types of HK 
      deficiency, one in which the HK activity was reduced exclusively in the RBC (HKR 
      defect) and another with general decrease of HK activity in several tissues (HKI 
      defect).
FAU - Murakami, K
AU  - Murakami K
AD  - Division of Pediatric Hematology, College of Physicians & Surgeons of Columbia 
      University, New York 10032, USA.
FAU - Piomelli, S
AU  - Piomelli S
LA  - eng
GR  - R01 HL48996-15/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
CIN - Blood. 1998 Jan 1;91(1):363-4. PMID: 9414310
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA, Complementary/blood/*isolation & purification
MH  - Erythrocytes/*enzymology/metabolism
MH  - Hexokinase/*blood/*genetics/isolation & purification
MH  - Humans
MH  - Isoenzymes/*blood/*genetics/isolation & purification
MH  - Leukemia, Erythroblastic, Acute/enzymology/genetics
MH  - Molecular Sequence Data
MH  - RNA, Messenger/biosynthesis
MH  - Reticulocytes/enzymology/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0006-4971(20)58877-0 [pii]
PST - ppublish
SO  - Blood. 1997 Feb 1;89(3):762-6.