PMID- 9029069
OWN - NLM
STAT- MEDLINE
DCOM- 19970312
LR  - 20061115
IS  - 0364-5134 (Print)
IS  - 0364-5134 (Linking)
VI  - 41
IP  - 2
DP  - 1997 Feb
TI  - Sequestration of RNA in Alzheimer's disease neurofibrillary tangles and senile 
      plaques.
PG  - 200-9
AB  - The polypeptide composition of neurofibrillary tangles (NFTs) and senile plaques 
      (SPs) has been characterized extensively within the Alzheimer's disease (AD) 
      brain. Because few data exist on the nonproteinaceous components of these 
      lesions, we sought to determine if NFTs, neuropil threads (NTs), and SPs contain 
      RNA species. To accomplish this, acridine orange (AO) histofluorescence was 
      employed, alone or in combination with thioflavine S (TS) staining and 
      immunohistochemistry to identify RNAs in paraffin-embedded tissue sections of 
      hippocampus and entorhinal cortex. Postmortem brain samples came from 32 subjects 
      including AD and elderly Down's syndrome (DS) patients, age-matched normal 
      controls, and non-AD diseased controls. AO stained the cytoplasm of normal 
      hippocampal and entorhinal neurons in all of the cases, while NFTs, NTs, and SPs 
      were AO-positive in the same regions of AD and DS brains. Cytoplasmic AO 
      histofluorescence was abolished with RNase, but not DNase or proteinase K, 
      indicating the relative specificity of AO for RNA species. Quantitative analysis 
      of double-labeled sections demonstrated that approximately 80% of TS-positive 
      NFTs also were AO-positive, whereas approximately 55% of TS-stained SPs contained 
      AO labeling. These novel observations demonstrate the presence of RNAs in NFTs, 
      NTs, and SPs.
FAU - Ginsberg, S D
AU  - Ginsberg SD
AD  - Department of Pathology and Laboratory Medicine, University of Pennsylvania 
      School of Medicine, Philadelphia 19104-4283, USA.
FAU - Crino, P B
AU  - Crino PB
FAU - Lee, V M
AU  - Lee VM
FAU - Eberwine, J H
AU  - Eberwine JH
FAU - Trojanowski, J Q
AU  - Trojanowski JQ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Neurol
JT  - Annals of neurology
JID - 7707449
RN  - 0 (tau Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alzheimer Disease/*pathology
MH  - Female
MH  - Hippocampus/*pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neurofibrillary Tangles/*pathology
MH  - RNA/metabolism
MH  - tau Proteins/*metabolism
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1002/ana.410410211 [doi]
PST - ppublish
SO  - Ann Neurol. 1997 Feb;41(2):200-9. doi: 10.1002/ana.410410211.