PMID- 9029669
OWN - NLM
STAT- MEDLINE
DCOM- 19970528
LR  - 20131121
IS  - 1078-0297 (Print)
IS  - 1078-0297 (Linking)
VI  - 94
IP  - 3
DP  - 1996 Dec
TI  - Renal tubular epithelial cell-E. coli interaction products stimulate nitric oxide 
      production in cultured rat renal medullary interstitial and mesangial cells.
PG  - 227-38
AB  - Tubulointerstitial and periglomerular inflammation and fibrosis are important 
      consequences of pyelonephritis. The pathogenesis of these abnormalities is not 
      fully understood. Renal tubular epithelial cells (RTEC) elaborate biologically 
      active materials following incubation with bacteria. Nitric oxide (NO) is an 
      inflammatory mediator and it modulates the accumulation of extracellular matrix 
      proteins. Therefore, we studied whether RTEC-E. coli interaction products 
      regulate NO production by cultured rat renal medullary interstitial cells (RMIC) 
      and mesangial cells (MC). RMIC and MC were maintained in media containing 
      IFN-gamma and LPS for 24-72 h. Test media contained either no further additives 
      or 20% supernatants from RTEC incubated with E. coli or bacterial cell products. 
      RTEC-E. coli interaction products significantly increased NO production in RMIC 
      and MC. This stimulation in NO production was not associated with changes in 
      inducible nitric oxide synthase (iNOS) gene or protein expression. These findings 
      indicate that RTEC-E. coli interaction products increase NO production in RMIC 
      and MC by directly stimulating iNOS enzymatic activity. Altered NO production by 
      renal cells may contribute to tubulointerstitial inflammation in acute and 
      chronic pyelonephritis.
FAU - Trachtman, H
AU  - Trachtman H
AD  - Department of Pediatrics, Schneider Children's Hospital, New Hyde Park, NY 
      11040-1432, USA. trachtma@lij.edu
FAU - Futterweit, S
AU  - Futterweit S
FAU - Singhal, P C
AU  - Singhal PC
FAU - Sankaran, R
AU  - Sankaran R
FAU - Franki, N
AU  - Franki N
LA  - eng
GR  - 2-RO1-DA06753-05/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Res Commun Mol Pathol Pharmacol
JT  - Research communications in molecular pathology and pharmacology
JID - 9437512
RN  - 0 (Culture Media)
RN  - 0 (Lipopolysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Communication/*physiology
MH  - Culture Media
MH  - Epithelium/metabolism
MH  - Escherichia coli/*metabolism
MH  - Glomerular Mesangium/*metabolism
MH  - Interferon-gamma/pharmacology
MH  - Kidney Medulla/*metabolism
MH  - Kidney Tubules/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Nitric Oxide/*biosynthesis
MH  - Rats
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Res Commun Mol Pathol Pharmacol. 1996 Dec;94(3):227-38.