PMID- 9029785
OWN - NLM
STAT- MEDLINE
DCOM- 19970414
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 46
IP  - 1-2
DP  - 1996 Dec
TI  - Identification and analyses of glycoprotein B of human herpesvirus 7.
PG  - 125-37
AB  - The gene for the human herpes virus 7 (HHV-7) glycoprotein B (gB) has been 
      identified by sequencing a molecularly cloned HHV-7 DNA fragment. A 2.5-kb open 
      reading frame (ORF) encoded a protein of 822 amino acids with characteristics of 
      a transmembrane glycoprotein, and showed the strongest similarity (56.5%) with 
      the human herpesvirus 6 (HHV-6) gB. The genes for the transport/capsid assembly 
      protein (tp/cap) and the DNA polymerase (pol) existed upstream and downstream of 
      the gB gene, respectively. This arrangement was the same as that of HHV-6. 
      Antisera were generated by immunizing mice with a glutathione 
      S-transferase-carboxy terminal gB fusion protein. Immunofluorescent tests 
      demonstrated that the antisera reacted specifically with HHV-7 antigens in 
      cytoplasm of infected cells. The antisera immunoprecipitated proteins with 
      apparent molecular masses of 51, 63 and 112 kDa from HHV-7 infected cells by 
      pulse-chase analysis. In the presence of tunicamycin, the protein with a 
      molecular mass of 112 kDa was replaced by a protein with a molecular mass of 88 
      kDa, and this size was consistent with the predicted size of the primary 
      translation product of the HHV-7 gB gene. These results suggested that the 
      protein with a molecular mass of 112 kDa was a glycoprotein synthesized by 
      addition of N-linked oligosaccharides to a non-glycosylated precursor of the 
      protein with a molecular mass of 88 kDa and then cleaved into the proteins with 
      molecular masses of 51 and 63 kDa in HHV-7 infected cells.
FAU - Hata, A
AU  - Hata A
AD  - Department of Virology, Osaka University, Japan.
FAU - Mukai, T
AU  - Mukai T
FAU - Isegawa, Y
AU  - Isegawa Y
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
SI  - GENBANK/D86577
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Protein Precursors)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein B, human herpesvirus 7)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Herpesvirus 7, Human/*chemistry/genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Protein Precursors/chemistry/genetics/isolation & purification
MH  - Sequence Analysis, DNA
MH  - Viral Envelope Proteins/chemistry/genetics/*isolation & purification
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
AID - S0168-1702(96)01395-0 [pii]
AID - 10.1016/s0168-1702(96)01395-0 [doi]
PST - ppublish
SO  - Virus Res. 1996 Dec;46(1-2):125-37. doi: 10.1016/s0168-1702(96)01395-0.