PMID- 9030872
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20190512
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 107
IP  - 2
DP  - 1997 Feb
TI  - Immunoprecipitation of steroidogenic enzyme autoantigens with autoimmune 
      polyglandular syndrome type I (APS I) sera; further evidence for independent 
      humoral immunity to P450c17 and P450c21.
PG  - 335-40
AB  - Three steroidogenic P450 cytochromes, steroid 17alpha-hydroxylase (P450c17), 
      steroid 21-hydroxylase (P450c21) and side-chain cleavage enzyme (P450scc), have 
      been described as autoantigens in APS I. In this study we report an 
      immunoprecipitation assay for the detection of autoantibodies to these three 
      enzymes using in vitro 35S-labelled antigens. Overall, 33 out of 46 (72%) 
      patients with APS I had autoantibodies to at least one of the three proteins and 
      each protein was recognized by patient sera with equal frequency. A higher rate 
      of autoantibody positivity was observed in APS I patients with Addison's disease 
      compared with patients without Addison's disease (85% versus 39%). All 11 
      patients with ovarian failure had anti-P450c17 or anti-P450scc antibodies. The 
      immunoprecipitation results with P450c17, P450c21 and P450scc correlated well 
      with the results obtained by immunoblotting assays. In addition, the 
      steroidogenic enzymes 11beta-hydroxylase (P450c11beta), aromatase (P450arom), 
      3beta-hydroxysteroid dehydrogenase (3betaHSD) and adrenodoxin were studied by 
      immunoprecipitation assay, but no reaction was found either with 46 APS I or with 
      26 healthy control sera. To study the suggested immunological cross-reactivity 
      between P450c17 and P450c21 enzymes, nine APS I patient sera were preabsorbed 
      with bacterially expressed P450c17 or P450c21 and subsequently used in 
      immunoprecipitation assay. The absorption experiments clearly indicated that the 
      preincubation inhibited only the reactivity of corresponding antigen, suggesting 
      independent autoantibody response to the two enzymes. Our results suggest that 
      the immune response to some but not to all steroidogenic enzymes is a specific 
      feature of APS I that may be pathogenically significant.
FAU - Peterson, P
AU  - Peterson P
AD  - Institute of Medical Technology, University of Tampere, Finland.
FAU - Uibo, R
AU  - Uibo R
FAU - Peranen, J
AU  - Peranen J
FAU - Krohn, K
AU  - Krohn K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - EC 1.14.14.16 (Steroid 21-Hydroxylase)
RN  - EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
CIN - Clin Exp Immunol. 1997 Feb;107(2):227-9. PMID: 9030856
MH  - Absorption
MH  - Antibody Formation
MH  - Autoantibodies/metabolism
MH  - Autoantigens/*blood
MH  - Cholesterol Side-Chain Cleavage Enzyme/immunology
MH  - Humans
MH  - Immunoblotting
MH  - Polyendocrinopathies, Autoimmune/*blood/*immunology
MH  - Precipitin Tests
MH  - Steroid 17-alpha-Hydroxylase/immunology
MH  - Steroid 21-Hydroxylase/immunology
PMC - PMC1904569
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
PMCR- 1998/02/01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PHST- 1998/02/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2249.1997.282-ce1175.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1997 Feb;107(2):335-40. doi: 
      10.1111/j.1365-2249.1997.282-ce1175.x.