PMID- 9031459
OWN - NLM
STAT- MEDLINE
DCOM- 19970429
LR  - 20071114
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 77
IP  - 1
DP  - 1997 Jan
TI  - Rapid direct determination of low and high molecular weight kininogen in human 
      plasma by Particle Concentration Fluorescence Immunoassay (PCFIA).
PG  - 109-18
AB  - In this study, we employed Particle Concentration Fluorescence Immunoassay 
      (PCFIA), for directly measuring both high and low molecular weight kininogens (HK 
      and LK) in human plasma. In 38 normal donors, the mean values for plasma 
      kininogens were 93 mg/ml +/- 19 SD, 82 mg/ml +/- 12 SD, and 175 mg/ml +/- 29 SD, 
      respectively for LK, HK, and TotK (the sum of LK and HK detected by their common 
      heavy chains). Plasma completely deficient in HK and LK was unreactive (< 0.25 
      mg/ml) in all 3 assays whereas plasma from a patient with Fitzgerald Trait had an 
      HK value of 11 mg/ml, an LK value of 36 mg/ml and a TotK value of 59 mg/ml. The 
      reagents can be prepared in advance and all three kininogen determinations can be 
      performed, using the same diluted sample on 24 plasma samples, in triplicate, or 
      40 plasma samples, in duplicate, in less than 1 h. By performing all 3 kininogen 
      determinations, it is possible to differentiate cleaved from intact kininogens. 
      This technique will facilitate the widespread screening of kininogen levels in 
      biological fluids of normal humans as well as of patients with various diseases.
FAU - Scott, C F
AU  - Scott CF
AD  - Temple University School of Medicine, Sol Sherry Thrombosis Research Center, 
      Philadelphia, PA 19140, USA.
FAU - Shull, B
AU  - Shull B
FAU - Muller-Esterl, W
AU  - Muller-Esterl W
FAU - Colman, R W
AU  - Colman RW
LA  - eng
GR  - HL 45486/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Kininogens)
SB  - IM
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - Kininogens/*blood/immunology
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1997 Jan;77(1):109-18.