PMID- 9031614
OWN - NLM
STAT- MEDLINE
DCOM- 19970304
LR  - 20190920
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 74
IP  - 1
DP  - 1997 Jan
TI  - Clonal CD5-positive B lymphocytes in myelodysplastic syndrome with systemic 
      vasculitis and trisomy 8.
PG  - 37-40
AB  - Bone marrow and peripheral blood from a myelodysplastic syndrome (MDS) patient 
      with trisomy 8 and associated systemic vasculitis was investigated for clonal 
      lymphoid lineage involvement using simultaneous metaphase and interphase 
      fluorescence in situ hybridization (FISH) and immunocytochemistry with antibodies 
      against CD13 (granulocytic), glycophorin A (GPA, erythroid), and the lymphocytic 
      antigens CD3. CD5, CD20, and CD22. Trisomy 8 was detected in 55% of CD13+, 40% of 
      GPA+, 6% of CD5+, and 5% of CD20/22+, but not in CD3+ cells. In a complementary 
      experiment using interphase FISH on bone marrow cells sorted by flow cytometry, 
      13% of CD5/CD19 double-positive cells (76% purity) were found to be trisomic. The 
      results indicate the existence of a small CD5-positive B-lymphoid clone as part 
      of the MDS process in this patient. Since CD5/19-positive cells have been 
      proposed to be autoantibody producing, this finding might be a clue to the 
      pathogenesis underlying the propensity for MDS patients to develop 
      immune-mediated complications.
FAU - Billstrom, R
AU  - Billstrom R
AD  - Department of Medicine, University Hospital, Lund, Sweden.
FAU - Johansson, B
AU  - Johansson B
FAU - Strombeck, B
AU  - Strombeck B
FAU - el-Rifai, W
AU  - el-Rifai W
FAU - Larramendy, M
AU  - Larramendy M
FAU - Olofsson, T
AU  - Olofsson T
FAU - Mitelman, F
AU  - Mitelman F
FAU - Knuutila, S
AU  - Knuutila S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (CD5 Antigens)
SB  - IM
MH  - B-Lymphocytes/*immunology
MH  - Bone Marrow Cells
MH  - CD5 Antigens/*analysis
MH  - *Chromosomes, Human, Pair 8
MH  - Clone Cells
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Lymphocyte Subsets
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/complications/*genetics/*pathology
MH  - Trisomy/*pathology
MH  - Vasculitis/*complications
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s002770050253 [doi]
PST - ppublish
SO  - Ann Hematol. 1997 Jan;74(1):37-40. doi: 10.1007/s002770050253.