PMID- 9032219
OWN - NLM
STAT- MEDLINE
DCOM- 19970311
LR  - 20131121
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 155
IP  - 2
DP  - 1997 Feb
TI  - Autocrine modulation of IL-8 production by sputum neutrophils in chronic 
      bronchial sepsis.
PG  - 726-31
AB  - We examined the activity of neutrophils isolated from sputum and blood of 
      subjects with chronic bronchial sepsis in terms of interleukin-8 (IL-8), 
      IL-1beta, and tumor necrosis factor-alpha (TNF alpha) production. In sputum, the 
      numbers of neutrophils correlated with the concentrations of these cytokines. 
      Sputum neutrophils constitutively secreted large amounts of IL-8, IL-1beta, and 
      TNF alpha. The patterns of secretion, however, differed from those of blood 
      neutrophils stimulated with bacterial lipopolysaccharide (LPS). Cytokine 
      secretion was time-dependent and was inhibited by cycloheximide and 
      dexamethasone. IL-10 inhibition of IL-8 production by sputum neutrophils was 
      significantly less than that noted with activated neutrophils. Antibody to 
      IL-1beta but not to TNF alpha, inhibited IL-8 secretion by sputum neutrophils, 
      contrasting with results found using blood neutrophils. Incubation of blood 
      neutrophils in sputum sol induced a similar cytokine secretion profile to that 
      following exposure to LPS. The inhibitory effects on IL-8 protein production 
      correlated with messenger RNA (mRNA) gene expression. These observations indicate 
      that neutrophils are a significant source of IL-8 in purulent sputum, and that an 
      autocrine loop involving IL-1beta maintains secretion within the bronchus lumen.
FAU - Pang, G
AU  - Pang G
AD  - Discipline of Pathology, Faculty of Medicine, University of Newcastle, New South 
      Wales, Australia.
FAU - Ortega, M
AU  - Ortega M
FAU - Zighang, R
AU  - Zighang R
FAU - Reeves, G
AU  - Reeves G
FAU - Clancy, R
AU  - Clancy R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Blood/drug effects/metabolism
MH  - Bronchiectasis/*metabolism
MH  - Chronic Disease
MH  - Cycloheximide/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Interleukin-1/biosynthesis
MH  - Interleukin-8/*biosynthesis
MH  - Neutrophils/drug effects/*metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/isolation & purification
MH  - Sputum/*metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1164/ajrccm.155.2.9032219 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 1997 Feb;155(2):726-31. doi: 
      10.1164/ajrccm.155.2.9032219.