PMID- 9033844
OWN - NLM
STAT- MEDLINE
DCOM- 19970319
LR  - 20141120
IS  - 0764-4469 (Print)
IS  - 0764-4469 (Linking)
VI  - 319
IP  - 11
DP  - 1996 Nov
TI  - [The role of the transforming growth factor beta 1 (TGF-beta 1) and of vascular 
      endothelial growth factor (VEGF) on the in vitro angiogenesis process].
PG  - 1003-10
AB  - We have studied the role of 2 exogenous cytokines, the TGF beta 1 and the VEGF, 
      on the in vitro angiogenesis process. Endothelial cells were plated on fibrin 
      matrices either with 2% or with 10% human serum in 199 medium. Forty-eight hours 
      later, with 10% human serum, the capillary-like network index (the percentage of 
      the culture area covered by the capillary network) was about 50%. Under these 
      culture conditions, when TGF-beta 1 or VEGF were added, the capillary-like 
      network index augmented and was nearing 75%. When the index is high, using 
      confocal microscopy, we show that hollow capillaries are formed. Moreover, the 
      addition of VEGF increased the kinetics of the capillary-like network formation. 
      With 2% human serum, 48 h after seeding the capillary-like network index was 
      about 75%. In this case, the addition of TGF-beta 1 decreased the network index, 
      whereas the addition of VEGF increased the kinetics of its formation. These in 
      vitro angiogenesis experiments show that the serological factors underline the 2 
      antagonist effects of TGF-beta 1, but have no detectable effects on the activator 
      effects on the VEGF.
FAU - Vailhe, B
AU  - Vailhe B
AD  - Laboratoire de bioenergetique, Universite Joseph-Fourier, Grenoble, France.
FAU - Tranqui, L
AU  - Tranqui L
LA  - fre
PT  - English Abstract
PT  - Journal Article
TT  - Role du facteur beta 1 de transformation cellulaire (TGF-beta 1) et du facteur de 
      croissance de l'endothelium vasculaire (VEGF) sur le processus d'angiogenese in 
      vitro.
PL  - France
TA  - C R Acad Sci III
JT  - Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie
JID - 8503078
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Cell Division/drug effects
MH  - Endothelial Growth Factors/*chemistry
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lymphokines/*chemistry
MH  - Microscopy, Confocal
MH  - Neovascularization, Physiologic/*drug effects
MH  - Transforming Growth Factor beta/*chemistry
MH  - Umbilical Veins/cytology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - C R Acad Sci III. 1996 Nov;319(11):1003-10.