PMID- 9034137
OWN - NLM
STAT- MEDLINE
DCOM- 19970303
LR  - 20200930
IS  - 0037-9727 (Print)
IS  - 0037-9727 (Linking)
VI  - 214
IP  - 2
DP  - 1997 Feb
TI  - Quantification of changes in transforming growth factor-beta1 gene expression in 
      experimental crescentic glomerulonephritis.
PG  - 180-6
AB  - Transforming growth factor-beta1 (TGFbeta1) has recently been identified as a 
      promoter of glomerular scarring in glomerulonephritis. The present studies 
      employed polymerase chain reaction (PCR)-based methods to quantify changes in 
      transcriptional activation of TGFbeta1 in experimental crescentic 
      glomerulonephritis. The disease was induced in the rat, and total RNA was 
      isolated from glomeruli at stages in which proliferation, crescent formation, and 
      scarring were prominent features. Total glomerular RNA was reverse transcribed 
      (RT) to cDNA, and RT products were subsequently analyzed by comparative and 
      competitive PCR. Enhanced TGFbeta1 expression was apparent at the onset of 
      proliferative glomerulonephritis and prominent during the phase of crescent 
      formation. The observations indicate that PCR-based methods allow quantitation of 
      changes in glomerular TGFbeta1 expression in the course of glomerulonephritis.
FAU - Lianos, E A
AU  - Lianos EA
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA.
FAU - Liu, J
AU  - Liu J
FAU - Guglielmi, K
AU  - Guglielmi K
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Soc Exp Biol Med
JT  - Proceedings of the Society for Experimental Biology and Medicine. Society for 
      Experimental Biology and Medicine (New York, N.Y.)
JID - 7505892
RN  - 0 (DNA Primers)
RN  - 0 (Transforming Growth Factor beta)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - DNA Primers/chemistry
MH  - Disease Models, Animal
MH  - *Gene Expression Regulation
MH  - Glomerulonephritis/*metabolism/pathology
MH  - Kidney Glomerulus/metabolism/pathology
MH  - Male
MH  - Polymerase Chain Reaction
MH  - RNA/isolation & purification
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta/*biosynthesis/*genetics
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.3181/00379727-214-44086 [doi]
PST - ppublish
SO  - Proc Soc Exp Biol Med. 1997 Feb;214(2):180-6. doi: 10.3181/00379727-214-44086.