PMID- 9036930
OWN - NLM
STAT- MEDLINE
DCOM- 19970313
LR  - 20190723
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 108
IP  - 3
DP  - 1997 Mar
TI  - Differential expression of the calpactin I subunits annexin II and p11 in 
      cultured keratinocytes and during wound repair.
PG  - 307-12
AB  - Transforming growth factor beta1 (TGF-beta1) is an important modulator of skin 
      morphogenesis and cutaneous wound repair. To gain insight into the mechanisms of 
      TGF-beta1 action in the skin, we used the differential display RT-PCR technique 
      to identify genes that are regulated by this factor in cultured human 
      keratinocytes. We obtained several partial cDNA clones. One of them was identical 
      to the 3'-end of p11, the small and regulatory subunit of the calpactin I complex 
      [(annexin II)2(p11)2]. RNase protection and northern blot analysis revealed 
      specific regulation of expression of both subunits of this heterotetrameric 
      protein (p11 and annexin II) by TGF-beta1 as well as by other growth factors, 
      although the time course and degree of induction or suppression were different 
      for each gene. Furthermore, we analyzed p11 and annexin II expression in normal 
      and wounded skin. Both p11 and annexin II mRNAs were found in the dermal and 
      epidermal compartments of normal human skin. Immunohistochemical studies 
      demonstrated the presence of p11 at equally high levels in all layers of normal 
      epidermis and in the hyper-proliferative epithelium at the wound edge. By 
      contrast, annexin II expression was high in the basal layer of normal epidermis 
      but low in the suprabasal layers and in the hyper-proliferative epithelium at the 
      wound edge, suggesting a differentiation-specific regulation of this calpactin I 
      subunit. The differential expression and regulation of p11 and annexin II 
      subunits in keratinocytes suggest the existence of different ratios of monomeric 
      versus p11-complexed annexin II that might be associated with different cellular 
      functions.
FAU - Munz, B
AU  - Munz B
AD  - Max-Planck-Institut fur Biochemie, Department of Virus Research, Martinsried, 
      Germany.
FAU - Gerke, V
AU  - Gerke V
FAU - Gillitzer, R
AU  - Gillitzer R
FAU - Werner, S
AU  - Werner S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Annexin A2)
RN  - 0 (Annexins)
RN  - 0 (Peptides)
RN  - 0 (S100 Proteins)
RN  - 0 (S100 calcium binding protein A10)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Annexin A2/*genetics
MH  - Annexins/*chemistry
MH  - Cells, Cultured
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratinocytes/cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peptides/*genetics
MH  - *S100 Proteins
MH  - Skin/chemistry/injuries
MH  - Transforming Growth Factor beta/pharmacology
MH  - Wound Healing/*genetics
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0022-202X(15)42830-1 [pii]
AID - 10.1111/1523-1747.ep12286470 [doi]
PST - ppublish
SO  - J Invest Dermatol. 1997 Mar;108(3):307-12. doi: 10.1111/1523-1747.ep12286470.