PMID- 9037167
OWN - NLM
STAT- MEDLINE
DCOM- 19970324
LR  - 20190822
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 33
IP  - 1
DP  - 1997 Jan
TI  - LhcaR1 of the red alga Porphyridium cruentum encodes a polypeptide of the LHCI 
      complex with seven potential chlorophyll a-binding residues that are conserved in 
      most LHCs.
PG  - 157-67
AB  - The accessory light-harvesting polypeptides associated with photosystem I (LHCI) 
      in Porphyridium cruentum bind chlorophyll a, zeaxanthin and beta-carotene. A cDNA 
      library of P. cruentum was screened with an antiserum specific to the LHCI 
      polypeptides, and an 0.9 kb fragment was identified as coding for an LHCI 
      polypeptide. This cDNA, which we named LhcaR1, has an open reading frame encoding 
      222 amino acid residues including a putative transit peptide of 28 amino acids. 
      Hydropathy analysis suggests that there are three transmembrane helices in the 
      mature polypeptide. Each of the amino acid residues that bind chlorophyll (six 
      residues) and serve in stabilizing the helices in higher-plant LHCs are conserved 
      in helices 1 and 3 of P. cruentum LhcaR1. The N-terminal flanking regions of 
      these two helices also show high sequence conservation with other LHCs. Helix 2 
      contains a seventh putative chlorophyll-binding site, but resembles helix 2 of 
      higher-plant LHCs to a lesser degree. A sequence motif of 11 residues found near 
      the N-terminus and in each of the three helices suggests the possibility that the 
      red algal LhcaR1 derives from a gene duplication. Polypeptides of the expected 
      molecular weight in six other red algae (Achrochaetium, Bangia, Callithamnion, 
      Cyanidium, Polysiphonia, Spermothamnion) were recognized by the antiserum to P. 
      cruentum LHCI, indicating a wide distribution of LHCI in rhodophytes.
FAU - Tan, S
AU  - Tan S
AD  - Department of Plant Biology, University of Maryland, College Park 20742, USA.
FAU - Cunningham, F X Jr
AU  - Cunningham FX Jr
FAU - Gantt, E
AU  - Gantt E
LA  - eng
SI  - GENBANK/U58679
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (Algal Proteins)
RN  - 0 (Chlorophyll Binding Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Light-Harvesting Protein Complexes)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - 0 (Photosystem I Protein Complex)
RN  - 0 (Recombinant Proteins)
RN  - 1406-65-1 (Chlorophyll)
RN  - YF5Q9EJC8Y (Chlorophyll A)
SB  - IM
MH  - *Algal Proteins
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - Chlorophyll/*metabolism
MH  - Chlorophyll A
MH  - Chlorophyll Binding Proteins
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - *Genes, Plant
MH  - *Light-Harvesting Protein Complexes
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Open Reading Frames
MH  - Photosynthetic Reaction Center Complex Proteins/*biosynthesis/chemistry/genetics
MH  - *Photosystem I Protein Complex
MH  - Protein Structure, Secondary
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Rhodophyta/*genetics/*metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1997/01/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1023/a:1005715528297 [doi]
PST - ppublish
SO  - Plant Mol Biol. 1997 Jan;33(1):157-67. doi: 10.1023/a:1005715528297.