PMID- 9040785
OWN - NLM
STAT- MEDLINE
DCOM- 19970313
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 75
IP  - 2-3
DP  - 1996
TI  - Allelic fusion of DNA topoisomerase II alpha and retinoic acid receptor alpha 
      genes in adriamycin-resistant p388 murine leukemia revealed by fluorescence in 
      situ hybridization.
PG  - 164-6
AB  - Fluorescence in situ hybridization (FISH) analysis of metaphase and decondensed 
      free chromatin fibers from Adriamycin (ADR)-sensitive and ADR-resistant murine 
      cells demonstrated a close juxtaposition of topoisomerase II alpha (Top2a) and 
      retinoic acid receptor alpha (Rara) genes in adjacent chromatin in the 
      drug-resistant cells, and a close but separate genetic proximity in normal murine 
      chromatin. This provides physical evidence that the chromosome 11 allelic 
      rearrangement resulting in a chimeric truncated Top2a/Rara transcript in the 
      ADR-resistant cells is due to a novel fusion of the Topo2a and Rara genes. This 
      is the first description of a Rara gene disruption in cells selected for 
      antineoplastic drug resistance.
FAU - Squire, J A
AU  - Squire JA
AD  - Department of Pathology and Laboratory Medicine, University of Toronto, Canada.
FAU - McPherson, J P
AU  - McPherson JP
FAU - Beatty, B G
AU  - Beatty BG
FAU - Goldenberg, G J
AU  - Goldenberg GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Poly-ADP-Ribose Binding Proteins)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 80168379AG (Doxorubicin)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
RN  - EC 5.99.1.3 (Top2a protein, mouse)
SB  - IM
MH  - *Alleles
MH  - Animals
MH  - Antibiotics, Antineoplastic/pharmacology
MH  - Antigens, Neoplasm
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - *DNA Topoisomerases, Type II/*genetics
MH  - DNA-Binding Proteins
MH  - Doxorubicin/*pharmacology
MH  - Drug Resistance, Neoplasm
MH  - In Situ Hybridization, Fluorescence
MH  - Isoenzymes/*genetics
MH  - Leukemia P388/drug therapy/*genetics
MH  - Mice
MH  - Poly-ADP-Ribose Binding Proteins
MH  - Receptors, Retinoic Acid/*genetics
MH  - Recombination, Genetic
MH  - Stem Cells
MH  - Tumor Cells, Cultured
EDAT- 1996/01/01 00:00
MHDA- 1996/01/01 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1996/01/01 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1159/000134470 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1996;75(2-3):164-6. doi: 10.1159/000134470.