PMID- 9041562
OWN - NLM
STAT- MEDLINE
DCOM- 19970512
LR  - 20190831
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 30
IP  - 3
DP  - 1997 Mar
TI  - Possible involvement of ADP-ribosylation of particular enzymes in cell death 
      induced by nitric oxide-donors in human neuroblastoma cells.
PG  - 305-11
AB  - To clarify the mechanisms of nitric oxide (NO)-induced cell death in human 
      neuronal cells, we examined effects of NO donors such as sodium nitroprusside 
      (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) on activities of 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and poly(ADP-ribose) polymerase 
      (PARP) in human neuroblastoma cell line, SH-SY5Y. SNP-induced 
      [32P]ADP-ribosylation of 113-kDa and 37-kDa proteins in SH-SY5Y cells. Treatment 
      with PARP inhibitors such as 3-aminobenzamide and 1,5-isoquinolinediol partially 
      prevented SNAP-induced cell death of SH-SY5Y. In purified GAPDH (37-kDa protein), 
      SNP- and SNAP-induced enhancement of [32P]ADP-ribosylation, and inhibition of 
      GAPDH activity. These results suggest that NO-induced cell death in human 
      neuroblastoma SH-SY5Y cells possibly involves in covalent modifications such as 
      ADP-ribosylation in PARP and GAPDH.
FAU - Kamoshima, W
AU  - Kamoshima W
AD  - Department of Neurobiology, Kyoto Pharmaceutical University, Japan.
FAU - Kitamura, Y
AU  - Kitamura Y
FAU - Nomura, Y
AU  - Nomura Y
FAU - Taniguchi, T
AU  - Taniguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Poly(ADP-ribose) Polymerase Inhibitors)
RN  - 169D1260KM (Nitroprusside)
RN  - 20762-30-5 (Adenosine Diphosphate Ribose)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - GNN1DV99GX (Penicillamine)
SB  - IM
MH  - Adenosine Diphosphate Ribose/*metabolism
MH  - *Apoptosis
MH  - DNA Fragmentation
MH  - Enzyme Inhibitors/pharmacology
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism
MH  - Humans
MH  - Neuroblastoma
MH  - Neurons/*metabolism
MH  - Nitric Oxide/*metabolism
MH  - Nitroprusside/pharmacology
MH  - Penicillamine/analogs & derivatives/pharmacology
MH  - Poly(ADP-ribose) Polymerase Inhibitors
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - S-Nitroso-N-Acetylpenicillamine
MH  - Tumor Cells, Cultured
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0197-0186(96)00091-5 [pii]
AID - 10.1016/s0197-0186(96)00091-5 [doi]
PST - ppublish
SO  - Neurochem Int. 1997 Mar;30(3):305-11. doi: 10.1016/s0197-0186(96)00091-5.