PMID- 9042203
OWN - NLM
STAT- MEDLINE
DCOM- 19970327
LR  - 20071114
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 16
IP  - 6B
DP  - 1996 Nov-Dec
TI  - A modified fluorescence in situ hybridization (FISH) technique.
PG  - 3435-7
AB  - We have modified and shortened the routine fluorescence in situ hybridization 
      (FISH) technique by using microwave or formalin pretreatment for artificially 
      aging and microwave heating only for dehydrating the regular chromosome 
      preparations. The all-human telomere probe (Oncor, Inc., Gaithersburg, MD) was 
      used on murine K-1735 clone X-21 melanoma cells for this purpose. The intensity 
      of signals obtained by our modified technique(s) was comparable to that obtained 
      by the routine procedure. However, pretreatment of slides with hydrogen peroxide 
      (H2O2) completely obliterates the FISH signals.
FAU - Multani, A S
AU  - Multani AS
AD  - Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Hopwood, V L
AU  - Hopwood VL
FAU - Pathak, S
AU  - Pathak S
LA  - eng
GR  - RRO-4999-01/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
SB  - IM
MH  - Animals
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Melanoma, Experimental/genetics
MH  - Mice
MH  - Tumor Cells, Cultured
EDAT- 1996/11/01 00:00
MHDA- 1996/11/01 00:01
CRDT- 1996/11/01 00:00
PHST- 1996/11/01 00:00 [pubmed]
PHST- 1996/11/01 00:01 [medline]
PHST- 1996/11/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1996 Nov-Dec;16(6B):3435-7.