PMID- 9042404
OWN - NLM
STAT- MEDLINE
DCOM- 19970321
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 373
IP  - 2
DP  - 1997 Feb 3
TI  - X-ray-sensitivity of lymphocytes of aplastic- and Diamond-Blackfan-anemia 
      patients as detected by conventional cytogenetic and chromosome painting 
      techniques.
PG  - 225-35
AB  - Frequencies of spontaneously occurring and X-ray induced, stable and unstable 
      types of chromosome aberrations in peripheral blood lymphocytes from two groups 
      of radiosensitive patients, i.e., aplastic anemia (AA) and Diamond-Blackfan 
      anemia (DBA), were determined. Two types of staining methods, i.e., chromosome 
      painting with two cocktails of chromosome-specific DNA libraries (Nos. 1, 3, X 
      and Nos. 2, 4 and 8), as well as conventional Giemsa staining, were employed. 
      Chromosome painting was done with single and multicolor fluorescence in situ 
      hybridization (FISH). The frequencies of spontaneously occurring chromosome 
      aberrations in AA and DBA patients were not significantly different from healthy 
      individuals. Hypersensitivity to X-rays was seen both in G0 as well as in G2 
      phase of the cell cycle in lymphocytes from AA and DBA patients, confirming our 
      earlier findings using micronucleus (DBA) and G2 radiosensitivity (AA) assays.
FAU - van Diemen, P C
AU  - van Diemen PC
AD  - MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden 
      University, The Netherlands.
FAU - Maasdam, D
AU  - Maasdam D
FAU - Darroudi, F
AU  - Darroudi F
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Anemia, Aplastic/*genetics
MH  - Cell Cycle
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/methods
MH  - Fanconi Anemia/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*radiation effects
MH  - Radiation Tolerance
MH  - Translocation, Genetic
EDAT- 1997/02/03 00:00
MHDA- 1997/02/03 00:01
CRDT- 1997/02/03 00:00
PHST- 1997/02/03 00:00 [pubmed]
PHST- 1997/02/03 00:01 [medline]
PHST- 1997/02/03 00:00 [entrez]
AID - S0027-5107(96)00201-1 [pii]
AID - 10.1016/s0027-5107(96)00201-1 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Feb 3;373(2):225-35. doi: 10.1016/s0027-5107(96)00201-1.