PMID- 9044844
OWN - NLM
STAT- MEDLINE
DCOM- 19970305
LR  - 20071115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 57
IP  - 4
DP  - 1997 Feb 15
TI  - Stable translocations detected by fluorescence in situ hybridization: a rapid 
      surrogate end point to evaluate the efficacy of a potentiator of tumor response 
      to radiotherapy.
PG  - 672-7
AB  - Testing potential modifiers of the response of tumors to radiation therapy 
      requires large, expensive, and time-consuming clinical trials. It would, 
      therefore, be of value to have a rapid surrogate end point of tumor response that 
      could be used to evaluate such modifiers. We here propose that radiation-induced 
      stable chromosome translocations measured by fluorescence in situ hybridization 
      (FISH) could fulfill this purpose. The assay requires that the ratio of nonlethal 
      stable translocations to lethal dicentric aberrations be unity and not change 
      with radiation dose and that radiation-induced stable translocations remain in 
      the tumor cell population essentially indefinitely after irradiation. We have 
      tested these assumptions with four human tumor cell lines in vitro at doses of 
      1-5 Gy and found them to be valid. We also modified the response to fractionated 
      irradiation of a human tumor xenograft in three different ways and quantitated 
      the tumor response using clonogenic cell survival and using the FISH stable 
      translocation assay. Both assays gave similar values for the extent of radiation 
      modification. These data suggest that this assay could allow clinical evaluation 
      of potential radiation sensitizers with fewer patients and in shorter times than 
      is the case with conventional clinical trials.
FAU - Kovacs, M S
AU  - Kovacs MS
AD  - Department of Radiation Oncology, Stanford University, California 94305, USA.
FAU - Yudoh, K
AU  - Yudoh K
FAU - Evans, J W
AU  - Evans JW
FAU - Menke, D
AU  - Menke D
FAU - Brown, J M
AU  - Brown JM
LA  - eng
GR  - CA 15201/CA/NCI NIH HHS/United States
GR  - CA 67166/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Animals
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - Immunologic Factors
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, SCID
MH  - Neoplasms/*genetics/*radiotherapy
MH  - Specific Pathogen-Free Organisms
MH  - Time Factors
MH  - *Translocation, Genetic
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1997/02/15 00:00
MHDA- 1997/02/15 00:01
CRDT- 1997/02/15 00:00
PHST- 1997/02/15 00:00 [pubmed]
PHST- 1997/02/15 00:01 [medline]
PHST- 1997/02/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1997 Feb 15;57(4):672-7.