PMID- 9046103
OWN - NLM
STAT- MEDLINE
DCOM- 19970326
LR  - 20161124
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 21
IP  - 1
DP  - 1997 Jan
TI  - Osteoblastic cells from rat long bone. II: Adhesion to substrata and integrin 
      expression in primary and propagated cultures.
PG  - 7-16
AB  - Propagation in vitro of rat tibial osteoblasts (ROB) is accompanied by increased 
      expression of the early osteogenic marker alkaline phosphatase (AP) and 
      maturation of the osteogenic phenotype. In order to establish the pattern of the 
      integrin expressed in ROB during progression to the mature osteoblastic 
      phenotype, we have used biosynthetic, immunoblotting and immunohistochemical 
      assays. We immunoprecipitated from osteoblasts, expanded for 1.5- and 
      7.5-doubling, alpha 5 beta 1, alpha v beta 3, alpha 3 beta 1, alpha 6 beta 1 and 
      alpha 1 beta 1 integrin heterodimers; furthermore beta 5, alpha 2 and alpha 4 
      chains were detected by immunoblots and indirect immunofluorescence. alpha v, 
      alpha 1, alpha 6 subunits in most cells, and beta 3 and beta 1 subunits in a 
      minority, were found to be associated with adhesion plaques in osteoblasts of 
      1.5-, 4.5- and 7.5-doubling grown in the presence of FCS, while all other 
      subunits stained diffusely all the cells. Adhesion to fibronectin (FN), laminin 
      (LN), collagen type I (COL I) and III(COL III) by ROB at different doubling 
      (1.5-11) was dependent on substratum concentration, and after 2.5 h at 55 nM 60% 
      of the cells adhered to all substrata. Arg-Gly-Asp-Ser (RGDS) containing peptides 
      inhibited adhesion of cells differentially, according to substratum; no 
      dependence on extent of progation in vitro was observed. In conclusion, ROB 
      cultured in vitro for 1.5- to 11-doubling had an unchanged pattern of expression 
      of integrin subunits, heterodimer association and cellular distribution. Adhesion 
      specificity and affinity were also unchanged. These results suggest that the 
      phenotypic maturation, detected as an increase in AP expression, is not 
      accompanied by major changes in the potential for cell-matrix interactions, and 
      does not correspond to changes in the type of integrin subunits expressed by 
      osteoblasts.
FAU - Castoldi, M
AU  - Castoldi M
AD  - Istituto di Fisiologia, Universita di Genova, Italy.
FAU - Pistone, M
AU  - Pistone M
FAU - Caruso, C
AU  - Caruso C
FAU - Puddu, A
AU  - Puddu A
FAU - Filanti, C
AU  - Filanti C
FAU - Piccini, D
AU  - Piccini D
FAU - Tacchetti, C
AU  - Tacchetti C
FAU - Manduca, P
AU  - Manduca P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Fibronectins)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
RN  - 0 (Oligopeptides)
RN  - 9001-32-5 (Fibrinogen)
RN  - 9007-34-5 (Collagen)
RN  - AC6UDA2MFC (arginyl-glycyl-aspartyl-serine)
SB  - IM
MH  - Animals
MH  - Cell Adhesion/drug effects/physiology
MH  - Cell Differentiation/physiology
MH  - Cell Division/physiology
MH  - Cells, Cultured/chemistry/cytology/metabolism
MH  - Collagen/pharmacology
MH  - Extracellular Matrix/chemistry
MH  - Fibrinogen/pharmacology
MH  - Fibronectins/pharmacology
MH  - Fluorescent Antibody Technique
MH  - Immunoblotting
MH  - Integrins/analysis/*biosynthesis/genetics
MH  - Laminin/pharmacology
MH  - Oligopeptides/pharmacology
MH  - Osteoblasts/*chemistry/*cytology
MH  - Phenotype
MH  - Rats
MH  - Rats, Wistar
MH  - Tibia/*cytology
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S1065-6995(96)90110-9 [pii]
AID - 10.1006/cbir.1996.0110 [doi]
PST - ppublish
SO  - Cell Biol Int. 1997 Jan;21(1):7-16. doi: 10.1006/cbir.1996.0110.