PMID- 9050098
OWN - NLM
STAT- MEDLINE
DCOM- 19970613
LR  - 20181130
IS  - 0300-0729 (Print)
IS  - 0300-0729 (Linking)
VI  - 34
IP  - 4
DP  - 1996 Dec
TI  - Fibroblasts but not epithelial cells obtained from human nasal mucosa produce the 
      chemokine RANTES.
PG  - 210-4
AB  - RANTES is a chemokine that was already found in tissues obtained from nasal 
      polyps of patients suffering from chronic polypous sinusitis. Its cellular origin 
      is as yet unknown. The aim of this study was to investigate whether human nasal 
      mucosa fibroblasts and epithelial cells are capable to produce RANTES. 
      Fibroblasts and epithelial cells, obtained from healthy human nasal mucosa, were 
      cultured. Expression of RANTES-mRNA and secretion of RANTES-protein in 
      supernatants was investigated after stimulation with 50 ng/ml Tumour Necrosis 
      Factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interferon-g 
      (IFN-gamma), lipopolysaccharide (LPS), phorbolymyristate acetate (PMA) and 
      serum-free medium (SFM) for 24 h. Cultivated nasal fibroblasts either expressed 
      RANTES-mRNA or secreted RANTES protein upon TNF-alpha, IL-1 beta and IFN-gamma 
      stimulation. The amounts of RANTES-protein production ranged from 23 ng/ml (PMA) 
      to 198 ng/ml (TNF-alpha). Nasal epithelial cells expressed RANTES-mRNA only after 
      stimulation with PMA. Secretion of significant amounts of RANTES protein were not 
      detected in the supernatants from nasal epithelial cells. We conclude that nasal 
      fibroblasts but not epithelial cells could be a cellular source of RANTES in 
      nasal mucosa or in secretions of patients suffering from diseases, where 
      eosinophilic tissue infiltration represents a characteristic histopathological 
      feature.
FAU - Maune, S
AU  - Maune S
AD  - Department of Otorhinolaryngology/Head and Neck Surgery, University of Kiel, 
      Germany.
FAU - Berner, I
AU  - Berner I
FAU - Sticherling, M
AU  - Sticherling M
FAU - Kulke, R
AU  - Kulke R
FAU - Bartels, J
AU  - Bartels J
FAU - Schroder, J M
AU  - Schroder JM
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Rhinology
JT  - Rhinology
JID - 0347242
RN  - 0 (Chemokine CCL5)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL5/*biosynthesis/genetics
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Fibroblasts/cytology/*metabolism
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Nasal Mucosa/cytology/*metabolism
MH  - RNA, Messenger/analysis
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1996/12/01 00:00
MHDA- 1996/12/01 00:01
CRDT- 1996/12/01 00:00
PHST- 1996/12/01 00:00 [pubmed]
PHST- 1996/12/01 00:01 [medline]
PHST- 1996/12/01 00:00 [entrez]
PST - ppublish
SO  - Rhinology. 1996 Dec;34(4):210-4.