PMID- 9051183
OWN - NLM
STAT- MEDLINE
DCOM- 19970515
LR  - 20161020
IS  - 0884-6812 (Linking)
VI  - 19
IP  - 1
DP  - 1997 Feb
TI  - Symmetric chromosomal order in leukocytes indicated by DNA image cytometry and 
      fluorescence in situ hybridization.
PG  - 30-6
AB  - OBJECTIVE: To study if the symmetry observed in the nuclear structure of 
      polymorphonuclear cells (PNC) could be verified functionally by determining DNA 
      and chromosome distributions using image cytometry (ICM) and fluorescence in situ 
      hybridization (FISH) STUDY DESIGN: In murine and human leukocytes the proportion 
      of PNCs with apparently bilaterally symmetric nuclei was determined. By ICM, 
      total nuclear DNA and DNA content in each half of these nuclei were measured. For 
      chromosomal localization the FISH technique was applied. RESULTS: About 13% of 
      PNC nuclei appeared to be bilaterally symmetric. ICM of Feulgen DNA revealed that 
      the PNC had a 2N value, similar to mononuclear cells. The DNA content in each of 
      the two symmetric parts of the nucleus was exactly the same. Similarly, FISH 
      signals specific to chromosomes 1 and 2 were distributed bilaterally on the two 
      symmetric parts. CONCLUSION: The distribution of DNA and chromosomes studied 
      support the hypothesis of bilateral symmetry of PNC nuclei, suggesting haploid 
      separation. This specific distribution may be valuable for the study of 
      mechanisms leading to DNA and chromosome damage by exposures to chemicals or 
      radiation and may have far-reaching significance in genetics regarding 
      imprinting, meiotic pairing and other subjects.
FAU - Chaudhuri, J P
AU  - Chaudhuri JP
AD  - Cancer Cytogenetics Unit, Ludwig Maximilian University, Munich, Federal Republic 
      of Germany.
FAU - Reith, A
AU  - Reith A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Quant Cytol Histol
JT  - Analytical and quantitative cytology and histology
JID - 8506819
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - *Chromosomes
MH  - Chromosomes, Human
MH  - DNA/*analysis/blood
MH  - Humans
MH  - Image Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocytes/chemistry/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Nasal Mucosa/chemistry/cytology
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
PST - ppublish
SO  - Anal Quant Cytol Histol. 1997 Feb;19(1):30-6.