PMID- 9052947
OWN - NLM
STAT- MEDLINE
DCOM- 19970313
LR  - 20031114
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 43
IP  - 4
DP  - 1996 Apr
TI  - Rapid and simultaneous detection of chromosome Y- and 1-bearing porcine 
      spermatozoa by fluorescence in situ hybridization.
PG  - 548-53
AB  - This study was carried out to develop a rapid and simultaneous detection system 
      of chromosome Y- and 1-bearing porcine spermatozoa by fluorescence in situ 
      hybridization (FISH). Chromosome Y- and 1-specific DNA probes were produced by 
      polymerase chain reaction with digoxigenin (Dig)- or biotin-dUTP. The 
      hybridization probe mixture of labeled Y-chromosome and chromosome 1-specific DNA 
      was applied to the preparation, immediately denatured at 75 degrees C for 8 min, 
      hybridized for 5 min at 37 degrees C and overall FISH steps were done within a 
      few hours. When double FISH with Dig-labeled chromosome Y-specific and 
      biotin-labeled chromosome 1-specific probes was applied to sperm nuclei 
      pretreated with dithiothreitol, the average of 50.9% of sperm nuclei had the 
      Dig-signal, 99.2% of the sperm nuclei had the biotin-signal and the average of 
      0.3% of sperm nuclei showed no signal. The putative rate of Y-bearing spermatozoa 
      ranged from 49.8% to 52.8% among 5 boars and the average putative rate of 
      Y-bearing spermatozoa was 51.0%. The results indicated that a rapid and 
      simultaneous FISH with chromosome Y- and 1-specific porcine DNA probes produced 
      by PCR made possible more accurate assessment of Y-bearing porcine spermatozoa.
FAU - Kawarasaki, T
AU  - Kawarasaki T
AD  - Shizuoka Swine and Poultry Experiment Station, Japan.
FAU - Sone, M
AU  - Sone M
FAU - Yoshida, M
AU  - Yoshida M
FAU - Bamba, K
AU  - Bamba K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Spermatozoa/*physiology
MH  - Swine
MH  - *Y Chromosome
EDAT- 1996/04/01 00:00
MHDA- 2000/06/20 09:00
CRDT- 1996/04/01 00:00
PHST- 1996/04/01 00:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1996/04/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2795(199604)43:4<548::AID-MRD18>3.0.CO;2-V [pii]
AID - 10.1002/(SICI)1098-2795(199604)43:4<548::AID-MRD18>3.0.CO;2-V [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1996 Apr;43(4):548-53. doi: 
      10.1002/(SICI)1098-2795(199604)43:4<548::AID-MRD18>3.0.CO;2-V.