PMID- 9055436
OWN - NLM
STAT- MEDLINE
DCOM- 19970401
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 63
IP  - 3
DP  - 1997 Mar
TI  - Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for 
      the presence of bai-like genes.
PG  - 1185-8
AB  - Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes 
      which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) 
      pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed 
      for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai 
      genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these 
      strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains 
      hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 
      12708.
FAU - Doerner, K C
AU  - Doerner KC
AD  - Department of Microbiology and Immunology, Virginia Commonwealth 
      University-Medical College of Virginia, Richmond 23298-0678, USA.
FAU - Takamine, F
AU  - Takamine F
FAU - LaVoie, C P
AU  - LaVoie CP
FAU - Mallonee, D H
AU  - Mallonee DH
FAU - Hylemon, P B
AU  - Hylemon PB
LA  - eng
GR  - 5 F32 DK08879/DK/NIDDK NIH HHS/United States
GR  - DK40986/DK/NIDDK NIH HHS/United States
GR  - P01 DK38030/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Cholic Acids)
RN  - G1JO7801AE (Cholic Acid)
SB  - IM
MH  - Blotting, Southern
MH  - Cholic Acid
MH  - Cholic Acids/*metabolism
MH  - Clostridium/*genetics/metabolism
MH  - Eubacterium/*genetics/metabolism
MH  - Feces/*microbiology
MH  - *Genes, Bacterial
MH  - Hydroxylation
PMC - PMC168411
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
PMCR- 1997/03/01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
PHST- 1997/03/01 00:00 [pmc-release]
AID - 10.1128/aem.63.3.1185-1188.1997 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1997 Mar;63(3):1185-8. doi: 
      10.1128/aem.63.3.1185-1188.1997.