PMID- 9055810
OWN - NLM
STAT- MEDLINE
DCOM- 19970407
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 185
IP  - 2
DP  - 1997 Feb 7
TI  - Structure, chromosomal localization and expression of mouse genes encoding type 
      III Reg, RegIII alpha, RegIII beta, RegIII gamma.
PG  - 159-68
AB  - Reg (regenerating gene), first isolated from a rat regenerating islet cDNA 
      library, is expressed in regenerating islet beta-cells. Recently, it has been 
      revealed that Reg and Reg-related genes constitute a multigene family, Reg 
      family, which consists of three subtypes (type I, II, III) based on the primary 
      structures of the encoded proteins of the genes. In mouse, type I and type II Reg 
      genes (i.e. RegI and RegII gene) have so far been isolated. In the present study, 
      the complete nucleotide (nt) sequences of the cDNAs and genes encoding murine 
      type III Reg (regenerating gene product), RegIII alpha, RegIII beta and RegIII 
      gamma were determined. RegIII alpha, RegIII beta and RegIII gamma encode 175-, 
      175- and 174-amino acid (aa) proteins, respectively, with 60-70% homology. All 
      three genes are composed of six exons and five introns spanning approx. 3 kb, and 
      exhibit distinctive structural features unique for members of the Reg gene 
      family. All the mouse Reg genes, RegIII alpha, RegIII beta, RegIII gamma, RegI 
      and RegII, are assigned to the adjacent site of chromosome 6C by fluorescence in 
      situ hybridization (FISH). RegIII alpha, RegIII beta and RegIII gamma were 
      expressed weakly in pancreas, strongly in intestinal tract, but not in 
      hyperplastic islets, whereas both RegI and RegII were expressed in hyperplastic 
      islets. These results suggest that genes of the mouse Reg family are derived from 
      a common ancestor gene by several gene duplications, and have obtained divergency 
      in expression and function in the process of genetic evolution.
FAU - Narushima, Y
AU  - Narushima Y
AD  - Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
FAU - Unno, M
AU  - Unno M
FAU - Nakagawara, K
AU  - Nakagawara K
FAU - Mori, M
AU  - Mori M
FAU - Miyashita, H
AU  - Miyashita H
FAU - Suzuki, Y
AU  - Suzuki Y
FAU - Noguchi, N
AU  - Noguchi N
FAU - Takasawa, S
AU  - Takasawa S
FAU - Kumagai, T
AU  - Kumagai T
FAU - Yonekura, H
AU  - Yonekura H
FAU - Okamoto, H
AU  - Okamoto H
LA  - eng
SI  - GENBANK/D63356
SI  - GENBANK/D63357
SI  - GENBANK/D63358
SI  - GENBANK/D63359
SI  - GENBANK/D63360
SI  - GENBANK/D63361
SI  - GENBANK/D63362
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Pancreatitis-Associated Proteins)
RN  - 0 (Proteins)
RN  - 0 (Reg3a protein, mouse)
RN  - 0 (Reg3g protein, mouse)
RN  - 0 (RegIII protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm
MH  - Base Sequence
MH  - Biomarkers, Tumor
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Evolution, Molecular
MH  - In Situ Hybridization, Fluorescence
MH  - Lectins, C-Type
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Pancreatitis-Associated Proteins
MH  - Promoter Regions, Genetic
MH  - Proteins/*genetics
MH  - Restriction Mapping
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Tissue Distribution
EDAT- 1997/02/07 00:00
MHDA- 1997/02/07 00:01
CRDT- 1997/02/07 00:00
PHST- 1997/02/07 00:00 [pubmed]
PHST- 1997/02/07 00:01 [medline]
PHST- 1997/02/07 00:00 [entrez]
AID - S0378111996005896 [pii]
AID - 10.1016/s0378-1119(96)00589-6 [doi]
PST - ppublish
SO  - Gene. 1997 Feb 7;185(2):159-68. doi: 10.1016/s0378-1119(96)00589-6.