PMID- 9056391
OWN - NLM
STAT- MEDLINE
DCOM- 19970407
LR  - 20161124
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 143
IP  - 2
DP  - 1997 Feb
TI  - Hydrogels containing peptide or aminosugar sequences implanted into the rat 
      brain: influence on cellular migration and axonal growth.
PG  - 287-99
AB  - Biocompatible polymer matrices for implantation into lesion sites in the brain 
      were synthesized by incorporating peptide or aminosugar sequences into 
      N-(2-hydroxypropyl)methacrylamide (HPMA) hydrogels. RGD peptide sequences were 
      chemically linked to the hydrogel backbone via a glycylglycine spacer; 
      aminosugars were glucosamine (NHGlc) or N-acetylglucosamine residues. Unmodified 
      or sequence containing HPMA hydrogels were implanted into the lesioned optic 
      tract or cerebral cortex of juvenile (17- to 19-day-old) or adult rat brains, 
      respectively. After 10-12 months host animals were perfused and the brains were 
      processed for immunohistochemistry using antibodies to neurofilaments (RT97), 
      laminin, glial fibrillary acidic protein (GFAP), carbonic anhydrase II (CAII), 
      S100 protein, macrophages (ED1), and myelin basic protein (MBP). Unmodified 
      (control) HPMA hydrogels contained no cellular infiltration or axonal growth. 
      Peptide (RGD)- and aminosugar-modified hydrogels showed increased adhesion 
      properties with host neural tissue, were vascularized, and were infiltrated by 
      host nonneuronal cells. Astrocytes (GFAP+) and macrophages (ED1(+)) were the 
      major cell types seen within modified HPMA hydrogels, the largest numbers being 
      found in RGD-containing polymers. CAII+ oligodendroglia were not seen within any 
      of the hydrogel matrices. RT97(+)/MBP- axons grew into both the RGD and NHGlc 
      hydrogel matrices for small distances. The number of axons was greatest in 
      hydrogels implanted into cerebral cortex but in both cortex and optic tract 
      implants the highest density of axons was seen in polymers containing RGD. The 
      findings of this study are discussed in the context of CNS tissue replacement and 
      the construction of bioactive scaffolds to promote regenerative axonal growth 
      across areas of injury in the brain and spinal cord.
FAU - Plant, G W
AU  - Plant GW
AD  - Department of Anatomy and Human Biology, The University of Western Australia, 
      Nedlands, Perth, WA, 6907, Australia.
FAU - Woerly, S
AU  - Woerly S
FAU - Harvey, A R
AU  - Harvey AR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - UKW89XAX2X (hydroxypropyl methacrylate)
SB  - IM
MH  - Animals
MH  - Axons/*drug effects
MH  - Brain/*metabolism
MH  - Cell Movement/*drug effects
MH  - Embryo Implantation/*immunology
MH  - Female
MH  - Methacrylates/*metabolism
MH  - Polymers/*pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - S0014-4886(97)96407-4 [pii]
AID - 10.1006/exnr.1997.6407 [doi]
PST - ppublish
SO  - Exp Neurol. 1997 Feb;143(2):287-99. doi: 10.1006/exnr.1997.6407.