PMID- 9056459
OWN - NLM
STAT- MEDLINE
DCOM- 19970417
LR  - 20171116
IS  - 0022-2011 (Print)
IS  - 0022-2011 (Linking)
VI  - 69
IP  - 2
DP  - 1997 Mar
TI  - A new tissue technique for evaluating effects of Bacillus thuringiensis toxins on 
      insect midgut epithelium.
PG  - 92-104
AB  - Epithelial tissue wholemounts were produced after enzymatic removal of basal 
      lamina and connective tissue from midguts of Trichoplusia ni larvae. Wholemounts 
      were nourished in artificial hemolymph and tissue viability was assessed for up 
      to 24 hr using the vital dyes trypan blue, acridine orange (AO), propidium iodide 
      (PI), and 4', 6-diamidino-2-phenylindole (DAPI). Peritrophic membrane synthesis 
      and modification of Bacillus thuringiensis Cry1Ac protoxin to active toxin 
      confirmed some normal epithelial function. Vital staining using the combination 
      of AO and PI, or DAPI revealed altered membrane permeability in columnar 
      epithelial and regenerative cells of tissues treated with activated Cry1Ac toxin 
      while feeding and oral inoculation bioassays verified Cry1Ac toxicity. DAPI was 
      selected to identify target cells in a rapid and highly sensitive assay.
FAU - Braun, L
AU  - Braun L
AD  - Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 
      0X2, Canada.
FAU - Keddie, B A
AU  - Keddie BA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (Bacillus thuringiensis protoxin)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Coloring Agents)
RN  - 0 (Protein Precursors)
SB  - IM
MH  - Animals
MH  - Bacillus thuringiensis/*metabolism
MH  - Bacterial Toxins/*analysis/pharmacology
MH  - Coloring Agents/analysis
MH  - Fluorescence
MH  - Host-Parasite Interactions
MH  - Insecta/*microbiology
MH  - Intestinal Mucosa/*chemistry/drug effects/pathology
MH  - Larva/microbiology
MH  - Lethal Dose 50
MH  - Protein Precursors/*analysis/pharmacology
MH  - Ultraviolet Rays
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - S0022-2011(96)94632-7 [pii]
AID - 10.1006/jipa.1996.4632 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 1997 Mar;69(2):92-104. doi: 10.1006/jipa.1996.4632.