PMID- 9056636
OWN - NLM
STAT- MEDLINE
DCOM- 19970626
LR  - 20081121
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 208
IP  - 3
DP  - 1997 Mar
TI  - Expression patterns of the four nuclear factor I genes during mouse embryogenesis 
      indicate a potential role in development.
PG  - 313-25
AB  - The nuclear factor I (NFI) family of site-specific DNA-binding proteins is 
      required for both the cell-type specific transcription of many viral and cellular 
      genes and for the replication of adenovirus DNA. Although binding sites for NFI 
      proteins within the promoters of several tissue-specific genes have been shown to 
      be essential for their expression, it is unclear which NFI gene products function 
      in specific tissues during development. We have isolated cDNAs from all four 
      murine NFI genes (gene designations Nfia, Nfib, Nfic, and Nfix), assessed the 
      embryonic and postnatal expression patterns of the NFI genes, and determined the 
      ability of specific NFI proteins to activate transcription from the NFI-dependent 
      mouse mammary tumor virus (MMTV) promoter. In adult mice, all four NFI genes are 
      most highly expressed in lung, liver, heart, and other tissues but only weakly 
      expressed in spleen and testis. The embryonic expression patterns of the NFI 
      genes is complex, with NFI-A transcripts appearing earliest-within 9 days 
      postcoitum in the heart and developing brain. The four genes exhibit unique but 
      overlapping patterns of expression during embryonic development, with high level 
      expression of NFI-A, NFI-B, and NFI-X transcripts in neocortex and extensive 
      expression of the four genes in muscle, connective tissue, liver, and other organ 
      systems. The four NFI gene products studied differ in their ability to activate 
      expression of the NFI-dependent MMTV promoter, with the NFI-B protein being most 
      active and the NFI-A protein being least active. These data are discussed in the 
      context of the developmental expression patterns of known NFI-responsive genes. 
      The differential activation of an NFI-dependent promoter, together with the 
      expression patterns observed for the four genes, indicate that the NFI proteins 
      may play an important role in regulating tissue-specific gene expression during 
      mammalian embryogenesis.
FAU - Chaudhry, A Z
AU  - Chaudhry AZ
AD  - Department of Cancer Biology, Research Institute, Cleveland Clinic Foundation, 
      Ohio 44195, USA.
FAU - Lyons, G E
AU  - Lyons GE
FAU - Gronostajski, R M
AU  - Gronostajski RM
LA  - eng
SI  - GENBANK/U57633
SI  - GENBANK/U57634
SI  - GENBANK/U57635
SI  - GENBANK/U57636
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of 
      Anatomists
JID - 9201927
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nfia protein, mouse)
RN  - 0 (Nfic protein, mouse)
RN  - 0 (Nfix protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nuclear Proteins
MH  - Time Factors
MH  - Tissue Distribution
MH  - Transcription Factors/genetics/metabolism
MH  - Transcriptional Activation
MH  - Tumor Cells, Cultured
MH  - Y-Box-Binding Protein 1
EDAT- 1997/03/01 00:00
MHDA- 2000/06/22 10:00
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1997/03/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0177(199703)208:3<313::AID-AJA3>3.0.CO;2-L [pii]
AID - 10.1002/(SICI)1097-0177(199703)208:3<313::AID-AJA3>3.0.CO;2-L [doi]
PST - ppublish
SO  - Dev Dyn. 1997 Mar;208(3):313-25. doi: 
      10.1002/(SICI)1097-0177(199703)208:3<313::AID-AJA3>3.0.CO;2-L.