PMID- 9057902
OWN - NLM
STAT- MEDLINE
DCOM- 19970404
LR  - 20201209
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 117
IP  - 2-3
DP  - 1997 Feb 28
TI  - Local lymph node activation and IgE responses in brown Norway and Wistar rats 
      after dermal application of sensitizing and non-sensitizing chemicals.
PG  - 229-34
AB  - The local lymph node assay (LLNA) and the IgE test in the mouse are proposed 
      models for predictive recognition of low molecular weight chemicals causing 
      IgE-mediated allergic airway reactions in man. Since rats are commonly used in 
      routine toxicity studies and a previous study (Arts et al. (1996) Food Chem. 
      Toxicol. 34, 55-62) has shown that several rat strains were found appropriate for 
      the LLNA, the suitability of the rat for the IgE test was examined in the present 
      study. Serum IgE concentrations were examined following topical exposure of Brown 
      Norway (BN) and Wistar rats to each of four chemicals with known diverse 
      sensitization potential in humans: trimellitic anhydride (TMA), a dermal and 
      respiratory sensitizer, dinitrochlorobenzene (DNCB), a dermal sensitizer with no 
      or limited potential to cause respiratory allergy; formaldehyde (FA), a skin 
      irritant and dermal sensitizer with equivocal evidence for respiratory 
      sensitizing potential; methyl salicylate (MS), a skin irritant devoid of 
      sensitizing properties. Of the four tested chemicals, only exposure to TMA 
      resulted in a significant increase in serum IgE concentration and this response 
      was only evoked in the high-IgE-responding BN rat. The latter two chemicals were 
      also tested for lymph node activation, in casu the ear-draining lymph nodes. FA 
      caused a dose-dependent activation of the draining lymph nodes whereas MS was 
      inactive. The results as obtained with TMA, DNCB and MS in the rat are in 
      agreement with human data. The results with FA though, indicate the need for 
      further studies of chemicals that have both irritant and sensitizing properties 
      at about similar concentrations or may act through non-IgE-mediated immune 
      mechanisms.
FAU - Arts, J H
AU  - Arts JH
AD  - TNO Nutrition and Food Research Institute, Toxicology Division, Zeist, The 
      Netherlands.
FAU - Droge, S C
AU  - Droge SC
FAU - Spanhaak, S
AU  - Spanhaak S
FAU - Bloksma, N
AU  - Bloksma N
FAU - Penninks, A H
AU  - Penninks AH
FAU - Kuper, C F
AU  - Kuper CF
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - 0 (Allergens)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Phthalic Anhydrides)
RN  - 0 (Salicylates)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 80T61EUU7H (trimellitic anhydride)
RN  - LAV5U5022Y (methyl salicylate)
SB  - IM
MH  - Administration, Cutaneous
MH  - Allergens/administration & dosage/*toxicity
MH  - Animals
MH  - Cell Count
MH  - Cell Division/drug effects
MH  - Dermatitis, Contact/etiology/immunology
MH  - Dinitrochlorobenzene/administration & dosage/*toxicity
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Formaldehyde/administration & dosage/*toxicity
MH  - Immunoglobulin E/blood/*drug effects/immunology
MH  - Lymph Nodes/*drug effects/immunology/pathology
MH  - Organ Size/drug effects
MH  - Phthalic Anhydrides/administration & dosage/*toxicity
MH  - Rats
MH  - Rats, Wistar
MH  - Salicylates/administration & dosage/*toxicity
MH  - Skin Tests
EDAT- 1997/02/28 00:00
MHDA- 1997/02/28 00:01
CRDT- 1997/02/28 00:00
PHST- 1997/02/28 00:00 [pubmed]
PHST- 1997/02/28 00:01 [medline]
PHST- 1997/02/28 00:00 [entrez]
AID - S0300-483X(96)03576-7 [pii]
AID - 10.1016/s0300-483x(96)03576-7 [doi]
PST - ppublish
SO  - Toxicology. 1997 Feb 28;117(2-3):229-34. doi: 10.1016/s0300-483x(96)03576-7.