PMID- 9060403 OWN - NLM STAT- MEDLINE DCOM- 19970522 LR - 20230320 IS - 0938-8990 (Print) IS - 0938-8990 (Linking) VI - 8 IP - 2 DP - 1997 Feb TI - Isolation of monochromosomal hybrids for mouse chromosomes 3, 6, 10, 12, 14, and 18. PG - 81-5 AB - Mouse/human somatic cell hybrids constitute a valuable resource for both genetic and physical mapping. In this report, we describe the production and characterization of a series of six monochromosomal hybrids generated by fusion of murine micro-cells with intact human recipient cells. The presence of each mouse chromosome was characterized by PCR analysis and the integrity of the mouse chromosome retained in the hybrids confirmed by fluorescence in situ hybridization (FISH) analysis. FAU - Sabile, A AU - Sabile A AD - Unite de Genetique Moleculaire Murine, Institut Pasteur, Paris, France. FAU - Poras, I AU - Poras I FAU - Cherif, D AU - Cherif D FAU - Goodfellow, P AU - Goodfellow P FAU - Avner, P AU - Avner P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Mamm Genome JT - Mammalian genome : official journal of the International Mammalian Genome Society JID - 9100916 SB - IM MH - Animals MH - Cell Line MH - *Chromosomes MH - Humans MH - *Hybrid Cells MH - In Situ Hybridization, Fluorescence MH - Mice MH - Polymerase Chain Reaction EDAT- 1997/02/01 00:00 MHDA- 1997/02/01 00:01 CRDT- 1997/02/01 00:00 PHST- 1997/02/01 00:00 [pubmed] PHST- 1997/02/01 00:01 [medline] PHST- 1997/02/01 00:00 [entrez] AID - 10.1007/s003359900362 [doi] PST - ppublish SO - Mamm Genome. 1997 Feb;8(2):81-5. doi: 10.1007/s003359900362.