PMID- 9060822
OWN - NLM
STAT- MEDLINE
DCOM- 19970407
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 150
IP  - 3
DP  - 1997 Mar
TI  - Allelic loss detected on chromosomes 8, 10, and 17 by fluorescence in situ 
      hybridization using single-copy P1 probes on isolated nuclei from 
      paraffin-embedded prostate tumors.
PG  - 841-50
AB  - We have implemented a reliable new technique for preparing isolated prostate 
      cancer nuclei from paraffin-embedded tissue sections followed by analysis with 
      single-copy fluorescence in situ hybridization (FISH). Our initial validation is 
      described by comparison of our data with fresh prostate tumor tissue and loss of 
      heterozygosity (LOH) studies. We also describe evaluation of 36 previously 
      unstudied prostate cancer patients. Fifteen archival samples were selected from 
      patients who underwent radical prostatectomy in which direct FISH and LOH data 
      were available. Isolated nuclei were prepared and allelic loss was detected on 
      17q using a single-copy DNA (P1 phage) probe by FISH. A high (80%) concordance 
      was found when comparing isolated nuclei data with 17q results from fresh 
      preparations and LOH studies. We also examined loss at sites on 8p, 10q, and 17q 
      in samples from 36 patients for whom clinical information was available. Loss was 
      found at any of the three loci in 32/36 (89%) of the specimens with specific loss 
      in 53% of the cases at the 8p locus, 33% at the 10q locus, and 61% at the 17q 
      locus. Studies indicate that, as well as providing potential clinical 
      information, isolated nuclei preparations are as reliable as fresh tissue for 
      single-copy FISH studies.
FAU - Deubler, D A
AU  - Deubler DA
AD  - Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake 
      City 84132, USA.
FAU - Williams, B J
AU  - Williams BJ
FAU - Zhu, X L
AU  - Zhu XL
FAU - Steele, M R
AU  - Steele MR
FAU - Rohr, L R
AU  - Rohr LR
FAU - Jensen, J C
AU  - Jensen JC
FAU - Stephenson, R A
AU  - Stephenson RA
FAU - Changus, J E
AU  - Changus JE
FAU - Miller, G J
AU  - Miller GJ
FAU - Becich, M J
AU  - Becich MJ
FAU - Brothman, A R
AU  - Brothman AR
LA  - eng
GR  - CA57183/CA/NCI NIH HHS/United States
GR  - CA59705/CA/NCI NIH HHS/United States
GR  - R01CA46269/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA Probes)
SB  - IM
CIN - Am J Pathol. 1997 Oct;151(4):1171-2. PMID: 9327751
MH  - Aged
MH  - Aged, 80 and over
MH  - *Alleles
MH  - Bacteriophage P1/genetics
MH  - Cell Nucleus/*genetics
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Retrospective Studies
PMC - PMC1857886
EDAT- 1997/03/01 00:00
MHDA- 1997/03/01 00:01
PMCR- 1997/09/01
CRDT- 1997/03/01 00:00
PHST- 1997/03/01 00:00 [pubmed]
PHST- 1997/03/01 00:01 [medline]
PHST- 1997/03/01 00:00 [entrez]
PHST- 1997/09/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1997 Mar;150(3):841-50.