PMID- 9061311
OWN - NLM
STAT- MEDLINE
DCOM- 19970610
LR  - 20190826
IS  - 0014-2972 (Print)
IS  - 0014-2972 (Linking)
VI  - 27
IP  - 2
DP  - 1997 Feb
TI  - Neutrophil superoxide and hydrogen peroxide production in patients with acute 
      liver failure.
PG  - 164-8
AB  - Defects in superoxide and hydrogen peroxide production may be implicated in the 
      high incidence of bacterial infections in patients with acute liver failure 
      (ALF). In the present study, oxygen radical production in patients with ALF due 
      to paracetamol overdose was compared with that of healthy volunteers. Neutrophils 
      from 14 ALF patients were stimulated via the complement receptors using zymosan 
      opsonized with ALF or control serum. Superoxide and hydrogen peroxide production 
      by ALF neutrophils stimulated with zymosan opsonized with ALF serum was 
      significantly reduced compared with the control subjects (P < 0.01). This defect 
      persisted when zymosan opsonized by control serum was used (P < 0.05). Superoxide 
      and hydrogen peroxide production in neutrophils stimulated with 
      formyl-methionyl-leucyl-phenylalanine (fMLP) from a further 18 ALF patients was 
      unaffected compared with control neutrophils. Serum C3 complement levels were 
      significantly reduced in ALF patients compared with control subjects (P < 
      0.0005). These results demonstrate a neutrophil defect in ALF due to paracetamol 
      overdose, that is complement dependent but independent of serum complement, 
      possibly connected to the complement receptor.
FAU - Clapperton, M
AU  - Clapperton M
AD  - Institute of Liver Studies, King's College Hospital, London, UK.
FAU - Rolando, N
AU  - Rolando N
FAU - Sandoval, L
AU  - Sandoval L
FAU - Davies, E
AU  - Davies E
FAU - Williams, R
AU  - Williams R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Clin Invest
JT  - European journal of clinical investigation
JID - 0245331
RN  - 0 (Complement C3)
RN  - 0 (Opsonin Proteins)
RN  - 11062-77-4 (Superoxides)
RN  - 362O9ITL9D (Acetaminophen)
RN  - 9010-72-4 (Zymosan)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Acetaminophen/adverse effects
MH  - Adolescent
MH  - Adult
MH  - Complement C3/chemistry/drug effects
MH  - Female
MH  - Humans
MH  - Hydrogen Peroxide/blood/*metabolism
MH  - Liver Failure, Acute/blood/chemically induced/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/*chemistry/*metabolism
MH  - Opsonin Proteins/metabolism
MH  - Statistics, Nonparametric
MH  - Superoxides/blood/*metabolism
MH  - Zymosan/immunology
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1046/j.1365-2362.1997.920640.x [doi]
PST - ppublish
SO  - Eur J Clin Invest. 1997 Feb;27(2):164-8. doi: 10.1046/j.1365-2362.1997.920640.x.