PMID- 9061912
OWN - NLM
STAT- MEDLINE
DCOM- 19970408
LR  - 20190914
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 40
IP  - 1
DP  - 1997 Feb
TI  - Use of meiotic FISH for identification of a new monosome in Gossypium hirsutum L.
PG  - 34-40
AB  - The extensive use of molecular cytogenetics in human genetics and clinical 
      diagnostics indicates that analogous applications in plants are highly feasible. 
      One sort of application would be the identification of new aneuploids, which 
      traditionally involves either direct karyotypic identification, which is feasible 
      in only a few plant species, or tests with markers (cytogenetic, genetic, or 
      molecular), which require sexual hybridization and at least one subsequent seed 
      or plant generation. We have used meiotic fluorescence in situ hybridization 
      (FISH) to analyze a new monosome of cotton (Gossypium hirsutum L., 2n = 4x = 52, 
      2(AD)1) that had a phenotype which seemed to be distinct from monosomes in the 
      Cotton Cytogenetic Collection. Painting with A2-genome DNA revealed the 
      monosome's D-subgenome origin. DAPI-PI staining showed that the monosome carries 
      a major NOR, delimiting it to the major NOR-bearing chromosomes of the 
      D-subgenome, i.e., 16 or 23. Dual-color FISH with 5S and 18S-28S rDNAs indicated 
      that the monosome contains separate major clusters of each of these two tandemly 
      repeated rDNA elements, thus delimiting the monosome to chromosome 23, for which 
      the Cotton Cytogenetic Collection has previously been devoid of any sort of 
      deficiency. Of the 26 chromosomes in the cotton genome, the Collection now 
      provides coverage for 16 (70%) in the form of monosomy, and 20 (77%) in the form 
      of monosomy and (or) telosomy. Use of molecular cytogenetic methods to identify a 
      new plant aneuploid in cotton exemplifies the fact that a physicochemical 
      karyotypic chromosome identification system is not required a priori for 
      application of new molecular cytogenetic methods, thus indicating their potential 
      applicability to nearly all plant species.
FAU - Ji, Y
AU  - Ji Y
AD  - Department of Soil and Crop Sciences, Texas A & M University, College Station 
      77843, USA. y0j1260@zeus.tamu.edu
FAU - Raska, D A
AU  - Raska DA
FAU - McKnight, T D
AU  - McKnight TD
FAU - Islam-Faridi, M N
AU  - Islam-Faridi MN
FAU - Crane, C F
AU  - Crane CF
FAU - Zwick, M S
AU  - Zwick MS
FAU - Hanson, R E
AU  - Hanson RE
FAU - Price, H J
AU  - Price HJ
FAU - Stelly, D M
AU  - Stelly DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 36015-30-2 (Propidium)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Fluorescent Dyes/chemistry
MH  - Genome, Plant
MH  - Gossypium/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/chemistry
MH  - Meiosis
MH  - *Monosomy
MH  - Nucleolus Organizer Region
MH  - Propidium/chemistry
MH  - Staining and Labeling
EDAT- 1997/02/01 00:00
MHDA- 1997/02/01 00:01
CRDT- 1997/02/01 00:00
PHST- 1997/02/01 00:00 [pubmed]
PHST- 1997/02/01 00:01 [medline]
PHST- 1997/02/01 00:00 [entrez]
AID - 10.1139/g97-005 [doi]
PST - ppublish
SO  - Genome. 1997 Feb;40(1):34-40. doi: 10.1139/g97-005.