PMID- 9062575
OWN - NLM
STAT- MEDLINE
DCOM- 19970326
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 93
IP  - 1
DP  - 1997 Jan
TI  - Molecular cytogenetic characterization of cancer cell alterations.
PG  - 10-21
AB  - Chromosomal abnormalities are the hallmark of cancer cells. Recurring and highly 
      consistent structural and numerical alterations have been identified in a large 
      number of leukemias, lymphomas, and solid tumors. The identification of recurrent 
      genetic alterations and the isolation of molecular markers have clinical 
      applications in the diagnosis and prognosis of neoplasia and in the detection of 
      minimal residual disease that are essential for designing the most effective 
      therapeutic approach. Polymerase chain reaction (PCR) and fluorescence in situ 
      hybridization (FISH) are powerful techniques for detection of genomic 
      alterations. The battery of FISH methods and DNA probes that are available can 
      resolve virtually any chromosomal alterations regardless of their complexity. 
      Combined chromosome banding, multifluor or spectral karyotype, and comparative 
      genomic hybridization (CGH) allow identification of structural and numerical 
      alterations on a global basis, mapping of the DNA copy number on the entire tumor 
      genome, complete derivation of complex rearrangements, and localization of the 
      breakpoints of translocations and deletions. Regions of recurrent alterations can 
      be microdisected, amplified, microclone libraries constructed and probes 
      localized on extended chromosomes or chromatin fibers for construction of high 
      resolution physical maps that are critical for positional cloning and gene 
      identification. In this review we attempted to cover the current trends in cancer 
      molecular cytogenetics, and to outline the importance of molecular chromosome 
      analysis in the understanding of oncogenesis and its clinical applications.
FAU - Popescu, N C
AU  - Popescu NC
AD  - Molecular Cytogenetics Section, National Cancer Institute, Bethesda, Maryland 
      20892, USA.
FAU - Zimonjic, D B
AU  - Zimonjic DB
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Chromosome Deletion
MH  - Chromosome Mapping
MH  - Gene Amplification
MH  - *Genetic Techniques
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasms/*genetics
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction/methods
MH  - Translocation, Genetic
MH  - Virus Integration
RF  - 109
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - S0165460896002622 [pii]
AID - 10.1016/s0165-4608(96)00262-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Jan;93(1):10-21. doi: 10.1016/s0165-4608(96)00262-2.