PMID- 9062704
OWN - NLM
STAT- MEDLINE
DCOM- 19970527
LR  - 20191101
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 72
IP  - 1
DP  - 1997 Jan
TI  - Immunohistochemical double staining of microwave enhanced and nonenhanced nuclear 
      and cytoplasmic antigens.
PG  - 10-5
AB  - Many of the antigens commonly investigated in histopathology can be enhanced by 
      microwave pretreatment (MWPT) of formalin fixed, paraffin embedded tissue 
      sections. We developed a double labeling method using microwave heating to detect 
      otherwise undetectable nuclear antigens combined with immunohistochemistry (IHC) 
      of cytoplasmic or membranous antigens that do not benefit from MWPT. We used the 
      same primary antibody solutions used in single antibody IHC. The staining 
      technique is based on the alkaline phosphatase anti-alkaline phosphatase (APAAP) 
      and the labeled avidin-biotin (LSAB) methods. Four different protocols were 
      tested, each modifying the sequence of MWPT, APAAP and LSAB staining. In this 
      study Ki67, estrogen receptor, progesterone receptor, c-neu, CD68 and desmin 
      primary antibodies were used in routinely formalin fixed, paraffin embedded 
      tissues of 50 tumor specimens. MWPT followed by LSAB for microwave enhanced 
      antigens and APAAP for antigens that cannot be enhanced by MWPT gave the best 
      double staining results. This method improves characterization of tumor cell 
      features from paraffin embedded tissue and should aid analysis of tumor 
      differentiation, receptor status and nuclear proteins in the single cells in 
      archival tissues.
FAU - Bohle, R M
AU  - Bohle RM
AD  - Department of Pathology, Justus-Liebig-University, Giessen, Germany.
FAU - Bonczkowitz, M
AU  - Bonczkowitz M
FAU - Altmannsberger, H M
AU  - Altmannsberger HM
FAU - Schulz, A
AU  - Schulz A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Antigens, Neoplasm/*analysis
MH  - Cell Nucleus/immunology
MH  - Cytoplasm/immunology
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - *Microwaves
MH  - Tumor Cells, Cultured
EDAT- 1997/01/01 00:00
MHDA- 1997/01/01 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/01/01 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.3109/10520299709082205 [doi]
PST - ppublish
SO  - Biotech Histochem. 1997 Jan;72(1):10-5. doi: 10.3109/10520299709082205.